Investigation of Anion-Exchange and Immunoaffinity Particle-Loaded Membranes for the Isolation of Charged Organic Analytes from Water

• Published in: Analytical chemistry (Washington) Vol. 70; no. 9; pp. 1969 - 1978
• Main Authors: Dombrowski, Tonya R, Wilson, George S, Thurman, E. Michael
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01-05-1998
• Subjects: Biological and medical sciences; Biotechnology; Chromatography, Affinity; Chromatography, Ion Exchange; Environment and pollution; Fundamental and applied biological sciences. Psychology; Immunochemistry; Industrial applications and implications. Economical aspects; Membranes, Artificial; Miscellaneous; Water Pollutants, Chemical - analysis; Water Supply - analysis; Performance evaluation; Aquatic environment; Analysis method; Immunoaffinity chromatography; Extraction; Anion exchange membrane; Ion exchange chromatography; Organic compounds
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac971081t

Anion-exchange and immunoaffinity particle loaded membranes (PLMs) were investigated as a mechanism for the isolation of charged organic analytes from water. Kinetic properties determined theoretically included dynamic capacity, pressure drop (ΔP), residence and diffusion times (T r, T d), and total membrane porosity (εT). These properties were confirmed through experimental evaluation, and the PLM method showed significant improvement over conventional solid-phase extraction (SPE) and ion-exchange formats. Recoveries of more than 90% were observed for a variety of test compounds at flow rates up to 70 mL/min (equipment-limited maximum flow rate). A fast-flow immunoaffinity column was developed using antibodies (Abs) attached to the PLMs. Reproducible recoveries (88% ± 4%) were observed at flow rates up to 70 mL/min for the antibody (Ab)-loaded PLMs. Findings indicate increased selectivity over anion-exchange PLMs and conventional SPE or ion-exchange methods and rapid Ab−antigen binding rates given the excellent mass-transfer characteristics of the PLMs.