Antioxidant Activity of Wines Determined by a Polarographic Assay Based on Hydrogen Peroxide Scavenge
Antioxidant (AO) activity of various red and white wines of different origin as well as some individual phenolic compounds present in wine has been assessed using a polarographic assay. Direct current polarography has been used to survey hydrogen peroxide scavenge (HPS) upon gradual addition of test...
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Published in: | Journal of agricultural and food chemistry Vol. 58; no. 8; pp. 4626 - 4631 |
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28-04-2010
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Abstract | Antioxidant (AO) activity of various red and white wines of different origin as well as some individual phenolic compounds present in wine has been assessed using a polarographic assay. Direct current polarography has been used to survey hydrogen peroxide scavenge (HPS) upon gradual addition of tested samples. Results expressed as reciprocal value of wine volume required for 50% decrease of anodic limiting current of hydrogen peroxide have been validated through correlation with Folin−Ciocalteau and DPPH assays. All wines exhibit HPS activity analogous with total phenolic content and DPPH scavenge. Reliability and accuracy, low cost, and rapid and direct experimental procedure open a wide area for application of this assay, making it a good alternative to standard, widely accepted AO assays. |
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AbstractList | Antioxidant (AO) activity of various red and white wines of different origin as well as some individual phenolic compounds present in wine has been assessed using a polarographic assay. Direct current polarography has been used to survey hydrogen peroxide scavenge (HPS) upon gradual addition of tested samples. Results expressed as reciprocal value of wine volume required for 50% decrease of anodic limiting current of hydrogen peroxide have been validated through correlation with Folin-Ciocalteau and DPPH assays. All wines exhibit HPS activity analogous with total phenolic content and DPPH scavenge. Reliability and accuracy, low cost, and rapid and direct experimental procedure open a wide area for application of this assay, making it a good alternative to standard, widely accepted AO assays. Antioxidant (AO) activity of various red and white wines of different origin as well as some individual phenolic compounds present in wine has been assessed using a polarographic assay. Direct current polarography has been used to survey hydrogen peroxide scavenge (HPS) upon gradual addition of tested samples. Results expressed as reciprocal value of wine volume required for 50% decrease of anodic limiting current of hydrogen peroxide have been validated through correlation with Folin−Ciocalteau and DPPH assays. All wines exhibit HPS activity analogous with total phenolic content and DPPH scavenge. Reliability and accuracy, low cost, and rapid and direct experimental procedure open a wide area for application of this assay, making it a good alternative to standard, widely accepted AO assays. |
Author | Gorjanović, Stanislava Z Potkonjak, Nebojša I Sužnjević, Desanka Z Novaković, Miroslav M |
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Keywords | polarography hydrogen peroxide scavenge DPPH total phenolics Antioxidant activity wine Bass Wine Edible fish Hydrogen Antioxidant Assay Alcoholic beverage Polarography Phenols Peroxides |
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Snippet | Antioxidant (AO) activity of various red and white wines of different origin as well as some individual phenolic compounds present in wine has been assessed... |
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SubjectTerms | Analytical Methods antioxidant activity Antioxidants - chemistry assays Biological and medical sciences Fermented food industries Fish and seafood industries food analysis food composition Food industries Free Radical Scavengers - chemistry Fundamental and applied biological sciences. Psychology hydrogen peroxide Hydrogen Peroxide - chemistry hydrogen peroxide scavenging phenolic compounds polarographic assays Wine wine quality wines Wines and vinegars |
Title | Antioxidant Activity of Wines Determined by a Polarographic Assay Based on Hydrogen Peroxide Scavenge |
URI | http://dx.doi.org/10.1021/jf100022e https://www.ncbi.nlm.nih.gov/pubmed/20302358 https://search.proquest.com/docview/733592524 |
Volume | 58 |
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