Investigation of Zinc Chelation in Zinc-Finger Arrays by Electrospray Mass Spectrometry
The chelation of zinc by consensus zinc-finger arrays of the CCCC, CCHC, and CCHH type has been investigated by electrospray ionization mass spectrometry. Accurate mass measurements of the most abundant isotopic species have demonstrated that two protons are lost for each Zn(II) ion chelated. Methyl...
Saved in:
| Published in: | Inorganic chemistry Vol. 38; no. 6; pp. 1322 - 1325 |
|---|---|
| Main Authors: | , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
American Chemical Society
22-03-1999
|
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The chelation of zinc by consensus zinc-finger arrays of the CCCC, CCHC, and CCHH type has been investigated by electrospray ionization mass spectrometry. Accurate mass measurements of the most abundant isotopic species have demonstrated that two protons are lost for each Zn(II) ion chelated. Methylation of zinc-finger peptides has revealed that two thiolate anions from cysteine side-chains are necessary to maintain chelation. The other cysteine(s) retain the thiol proton(s) and can be methylated without loss of chelating ability. |
|---|---|
| Bibliography: | istex:D2D56E60921F8B9D5FB7ED9CE7AD6A7C7C93D364 ark:/67375/TPS-6FJ3PMGJ-Q ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0020-1669 1520-510X |
| DOI: | 10.1021/ic9809963 |