Core–Shell Microfibers via Bioorthogonal Layer-by-Layer Assembly
A new technique is described for the construction of core–shell microfibers for biomedical applications. Fibrous scaffolds were fabricated by electrospinning, followed by covalent layer-by-layer deposition based on the rapid bioorthogonal reaction between s-tetrazines (Tz) and trans-cyclooctenes (TC...
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Published in: | ACS macro letters Vol. 9; no. 9; pp. 1369 - 1375 |
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American Chemical Society
15-09-2020
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Abstract | A new technique is described for the construction of core–shell microfibers for biomedical applications. Fibrous scaffolds were fabricated by electrospinning, followed by covalent layer-by-layer deposition based on the rapid bioorthogonal reaction between s-tetrazines (Tz) and trans-cyclooctenes (TCOs). Electrospun poly(ε-caprolactone) (PCL) scaffolds were subjected to surface modifications to install tetrazine groups. The scaffolds were iteratively submerged in aqueous solutions of TCO-modified hyaluronic acid (HA-TCO) and tetrazine-modified hyaluronic acid (HA-Tz), resulting in the controlled growth of a cross-linked HA gel around individual microfibers. Integrin-binding motifs were covalently attached to the surface of the microfibers using TCO-conjugated RGD peptide. The scaffolds fostered the attachment and growth of primary porcine vocal fold fibroblasts without a significant induction of the myofibroblast phenotype. Stimulation with transforming growth factor beta (TGF-β) moderately enhanced fibroblast activation, and inhibition of the Rho/ROCK signaling pathway using Y27632 further decreased the expression of myofibroblastic markers. The bioorthogonally assembled scaffolds with a stiff PCL core and a soft HA shell may find application as therapeutic implants for the treatment of vocal fold scarring. |
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AbstractList | A new technique is described for the construction of core-shell microfibers for biomedical applications. Fibrous scaffolds were fabricated by electrospinning, followed by covalent layer-by-layer deposition based on the rapid bioorthogonal reaction between
-tetrazines (Tz) and
-cyclooctenes (TCOs). Electrospun poly(ε-caprolactone) (PCL) scaffolds were subjected to surface modifications to install tetrazine groups. The scaffolds were iteratively submerged in aqueous solutions of TCO-modified hyaluronic acid (HA-TCO) and tetrazine-modified hyaluronic acid (HA-Tz), resulting in the controlled growth of a cross-linked HA gel around individual microfibers. Integrin-binding motifs were covalently attached to the surface of the microfibers using TCO-conjugated RGD peptide. The scaffolds fostered the attachment and growth of primary porcine vocal fold fibroblasts without a significant induction of the myofibroblast phenotype. Stimulation with transforming growth factor beta (TGF-β) moderately enhanced fibroblast activation, and inhibition of the Rho/ROCK signaling pathway using Y27632 further decreased the expression of myofibroblastic markers. The bioorthogonally assembled scaffolds with a stiff PCL core and a soft HA shell may find application as therapeutic implants for the treatment of vocal fold scarring. A new technique is described for the construction of core–shell microfibers for biomedical applications. Fibrous scaffolds were fabricated by electrospinning, followed by covalent layer-by-layer deposition based on the rapid bioorthogonal reaction between s-tetrazines (Tz) and trans-cyclooctenes (TCOs). Electrospun poly(ε-caprolactone) (PCL) scaffolds were subjected to surface modifications to install tetrazine groups. The scaffolds were iteratively submerged in aqueous solutions of TCO-modified hyaluronic acid (HA-TCO) and tetrazine-modified hyaluronic acid (HA-Tz), resulting in the controlled growth of a cross-linked HA gel around individual microfibers. Integrin-binding motifs were covalently attached to the surface of the microfibers using TCO-conjugated RGD peptide. The scaffolds fostered the attachment and growth of primary porcine vocal fold fibroblasts without a significant induction of the myofibroblast phenotype. Stimulation with transforming growth factor beta (TGF-β) moderately enhanced fibroblast activation, and inhibition of the Rho/ROCK signaling pathway using Y27632 further decreased the expression of myofibroblastic markers. The bioorthogonally assembled scaffolds with a stiff PCL core and a soft HA shell may find application as therapeutic implants for the treatment of vocal fold scarring. |
Author | Fox, Joseph M Ravikrishnan, Anitha Jia, Xinqiao Zhang, He |
AuthorAffiliation | University of Delaware Department of Chemistry and Biochemistry Department of Materials Science and Engineering |
AuthorAffiliation_xml | – name: University of Delaware – name: Department of Materials Science and Engineering – name: Department of Chemistry and Biochemistry |
Author_xml | – sequence: 1 givenname: Anitha orcidid: 0000-0002-2385-563X surname: Ravikrishnan fullname: Ravikrishnan, Anitha organization: Department of Materials Science and Engineering – sequence: 2 givenname: He surname: Zhang fullname: Zhang, He organization: Department of Materials Science and Engineering – sequence: 3 givenname: Joseph M orcidid: 0000-0002-8258-1640 surname: Fox fullname: Fox, Joseph M email: jmfox@udel.edu organization: University of Delaware – sequence: 4 givenname: Xinqiao orcidid: 0000-0002-3564-5576 surname: Jia fullname: Jia, Xinqiao email: xjia@udel.edu organization: Department of Materials Science and Engineering |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/35638624$$D View this record in MEDLINE/PubMed |
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CitedBy_id | crossref_primary_10_1016_j_biomaterials_2023_122180 crossref_primary_10_1016_j_bioactmat_2022_04_005 crossref_primary_10_1021_acs_biomac_2c00504 crossref_primary_10_3390_gels9120961 crossref_primary_10_1002_cplu_202300599 crossref_primary_10_1002_adhm_202203104 crossref_primary_10_1021_acsami_2c13852 |
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Snippet | A new technique is described for the construction of core–shell microfibers for biomedical applications. Fibrous scaffolds were fabricated by electrospinning,... A new technique is described for the construction of core-shell microfibers for biomedical applications. Fibrous scaffolds were fabricated by electrospinning,... |
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Title | Core–Shell Microfibers via Bioorthogonal Layer-by-Layer Assembly |
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