Simultaneous Multiharmonic Imaging of Nanoparticles in Tissues for Increased Selectivity

Simultaneous Multiharmonic Imaging of Nanoparticles in Tissues for Increased Selectivity

We investigate the use of bismuth ferrite (BFO) nanoparticles for tumor tissue labeling in combination with infrared multiphoton excitation at 1250 nm. We report the efficient and simultaneous generation of second- and third-harmonic signals by the nanoparticles. On this basis, we set up a novel ima...

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Bibliographic Details
Published in:ACS photonics Vol. 2; no. 10; pp. 1416 - 1422
Main Authors: Rogov, Andrii, Irondelle, Marie, Ramos Gomes, Fernanda, Bode, Julia, Staedler, Davide, Passemard, Solène, Courvoisier, Sébastien, Yamamoto, Yasuaki, Waharte, François, Ciepielewski, Daniel, Rideau, Philippe, Gerber-Lemaire, Sandrine, Alves, Frauke, Salamero, Jean, Bonacina, Luigi, Wolf, Jean-Pierre
Format: Journal Article
Language:English
Published: American Chemical Society 21-10-2015
Subjects:
second-harmonic generation
nanoparticles
multiphoton microscopy
third-harmonic generation
tissue imaging
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Summary:We investigate the use of bismuth ferrite (BFO) nanoparticles for tumor tissue labeling in combination with infrared multiphoton excitation at 1250 nm. We report the efficient and simultaneous generation of second- and third-harmonic signals by the nanoparticles. On this basis, we set up a novel imaging protocol based on the co-localization of the two harmonic signals and demonstrate its benefits in terms of increased selectivity against endogenous background sources in tissue samples. Finally, we discuss the use of BFO nanoparticles as mapping reference structures for correlative light–electron microscopy.
ISSN:2330-4022
2330-4022
DOI:10.1021/acsphotonics.5b00289