Metabolic Resistance to Protoporphyrinogen Oxidase-Inhibitor Herbicides in a Palmer amaranth Population from Kansas

• Published in: Journal of agricultural and food chemistry Vol. 72; no. 10; pp. 5122 - 5132
• Main Authors: Borgato, Ednaldo A., Thiagarayaselvam, Aarthy, Peterson, Dallas E., Hay, Marshall M., Dille, J. Anita, Jugulam, Mithila
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 13-03-2024
• Subjects: Agricultural and Environmental Chemistry; Amaranthus - metabolism; Benzamides; Halogenated Diphenyl Ethers; Herbicide Resistance - genetics; Herbicides - pharmacology; Kansas; Protoporphyrinogen Oxidase - genetics; Kansas; nontarget site resistance; fomesafen; cross-resistance; cytochrome P450 monooxygenases; lactofen
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.3c05333

Palmer amaranth has evolved target and nontarget site resistance to protoporphyrinogen oxidase-inhibitor herbicides in the United States. Recently, a population (KCTR) from a long-term conservation tillage study in Kansas was found to be resistant to herbicides from six sites of action, including to PPO-inhibitors, even with this herbicide group being minimally used in this field. This research investigated the level of resistance to postemergence PPO-inhibitors, target- and nontarget-site resistance mechanism(s), and efficacy of pre-emergence chemistries. The greenhouse experiments confirmed 6.1- to 78.9-fold resistance to lactofen in KCTR, with the level of resistance increasing when KCTR was purified for the resistance trait. PPO2 sequences alignment revealed the absence of known mutations conferring resistance to PPO-inhibitors in KCTR Palmer amaranth, and differential expression of the PPO2 gene did not occur. KCTR metabolized fomesafen faster than the susceptible population, indicating that herbicide detoxification is the mechanism conferring resistance in this population. Further, treatment with the cytochrome P450-inhibitor malathion followed by lactofen restored the sensitivity of KCTR to this herbicide. Despite being resistant to POST applied PPO-inhibitors, KCTR Palmer amaranth was completely controlled by the labeled rate of the PRE applied PPO-inhibitors fomesafen, flumioxazin, saflufenacil, sulfentrazone, and oxadiazon. The overall results suggest that P450-mediated metabolism confers resistance to PPO-inhibitors in KCTR, rather than alterations in the PPO2, which were more commonly found in other Palmer amaranth populations. Future work will focus on identifying the fomesafen metabolites and on unravelling the genetic basis of metabolic resistance to PPO-inhibitor herbicides in KCTR Palmer amaranth.