Mussel-Inspired Universal Bioconjugation of Polydiacetylene Liposome for Droplet-Array Biosensors

• Published in: ACS applied materials & interfaces Vol. 9; no. 48; pp. 42210 - 42216
• Main Authors: Kang, Do Hyun, Jung, Ho-Sup, Kim, Keesung, Kim, Jinsang
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 06-12-2017
• Subjects: Animals; Biosensing Techniques; Bivalvia; Liposomes; Polyacetylene Polymer - chemistry; Vascular Endothelial Growth Factor A; droplet-array biosensor; polydiacetylene liposome; dopamine; mussel-inspired chemistry; vascular endothelial growth factor detection
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.7b14086

Most solid-state biosensor platforms require a specific immobilization chemistry and a bioconjugation strategy separately to tether sensory molecules to a substrate and attach specific receptors to the sensory unit, respectively. We developed a mussel-inspired universal conjugation method that enables both surface immobilization and bioconjugation at the same time. By incorporating dopamine or catechol moiety into self-signaling polydiacetylene (PDA) liposomes, we demonstrated efficient immobilization of the PDA liposomes to a wide range of substrates, without any substrate modification. Moreover, receptor molecules having a specificity toward a target molecule can also be attached to the immobilized PDA liposome layer without any chemical modification. We applied our mussel-inspired conjugation method to a droplet-array biosensor by exploiting the hydrophilic nature of PDA liposomes coated on a hydrophobic polytetrafluoroethylene surface and demonstrated selective and sensitive detection of vascular endothelial growth factor down to 10 nM.