Expanded Coverage of the Human Heart Mitochondrial Proteome Using Multidimensional Liquid Chromatography Coupled with Tandem Mass Spectrometry

Recent evidence suggests that mitochondria are closely linked with the aging process and degenerative disorders such as Alzheimer's disease and Parkinson's disease. Thus, there has been increasing interest in cataloging mitochondrial proteomes to identify potential diagnostic and therapeut...

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Bibliographic Details
Published in:	Journal of proteome research Vol. 3; no. 3; pp. 495 - 505
Main Authors:	Gaucher, Sara P, Taylor, Steven W, Fahy, Eoin, Zhang, Bing, Warnock, Dale E, Ghosh, Soumitra S, Gibson, Bradford W
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 01-05-2004
Subjects:	Chromatography, Liquid Gas Chromatography-Mass Spectrometry Humans Mitochondria, Heart - metabolism Myocardium - metabolism Proteome Spectrometry, Mass, Electrospray Ionization
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Summary:	Recent evidence suggests that mitochondria are closely linked with the aging process and degenerative disorders such as Alzheimer's disease and Parkinson's disease. Thus, there has been increasing interest in cataloging mitochondrial proteomes to identify potential diagnostic and therapeutic targets. We have previously reported results of a one-dimensional electrophoresis/liquid chromatography MS/MS study to characterize the proteome of normal human heart mitochondria (Taylor et al. Nat. Biotechnol. 2003, 21, 281−286). We now report two subsequent studies where multidimensional liquid chromatography MS/MS was investigated as an alternative means for characterizing the same sample. Keywords: mitochondria • LC/MS/MS • MudPIT • MDLC
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1535-3893 1535-3907
DOI:	10.1021/pr034102a