Multicolored, Tb3+-Based Antibody-Free Detection of Multiple Tyrosine Kinase Activities

• Published in: Analytical chemistry (Washington) Vol. 87; no. 15; pp. 7555 - 7558
• Main Authors: Lipchik, Andrew M, Perez, Minervo, Cui, Wei, Parker, Laurie L
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 04-08-2015
• Subjects: Antibodies - chemistry; Biosensing Techniques; Color; Enzyme Assays - methods; Fluorescence; Protein-Tyrosine Kinases - metabolism; Terbium - chemistry
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.5b02233

Kinase signaling is a major mechanism driving many cancers. While many inhibitors have been developed and are employed in the clinic, resistance due to crosstalk and pathway reprogramming is an emerging problem. High-throughput assays to detect multiple pathway kinases simultaneously could better model these complex relationships and enable drug development to combat this type of resistance. We developed a strategy to take advantage of time-resolved luminescence of Tb3+-chelated phosphotyrosine-containing peptides, which facilitated efficient energy transfer to small molecule fluorophores conjugated to the peptides to produce orthogonally colored biosensors for two different kinases. This enabled multiplexed detection with high signal-to-noise in a high-throughput-compatible format. This proof-of-concept study provides a platform that could be applied to other lanthanide metal and fluorophore combinations to achieve even greater multiplexing without the need for phosphospecific antibodies.