Critical Secondary Metabolites Confer the Broad-Spectrum Pathogenic Fungi Resistance Property of a Marine-Originating Streptomyces sp. HNBCa1

• Published in: Journal of agricultural and food chemistry Vol. 72; no. 23; pp. 13164 - 13174
• Main Authors: Xiong, Zijun, Xia, Tengfei, Wu, Weicheng, Wang, Rong, Qi, Min, Zhang, Shiqing, Li, Jitao, Yang, Yang, Zheng, Daojun, Lin, Shuangjun, Guo, Zhikai
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 31-05-2024
• Subjects: Bioactive Constituents, Metabolites, and Functions; broad-spectrum resistance; secondary metabolites; pathogenic fungi; antifungal activity; biocontrol
• ISSN: 0021-8561; 1520-5118; 1520-5118
• DOI: 10.1021/acs.jafc.4c02805

Obtaining a microorganism strain with a broad-spectrum resistance property and highly efficient antifungal activity is important to the biocontrol strategy. Herein, a marine Streptomyces sp. HNBCa1 demonstrated a broad-spectrum resistance to 17 tested crop pathogenic fungi and exhibited a high biocontrol efficiency against mango anthracnose and banana fusarium wilt. To uncover the critical bioactive secondary metabolites basis, genome assembly and annotation, metabolomic analysis, and a semipreparative HPLC-based activity-guide method were employed. Finally, geldanamycin and ectoine involved in codifferential secondary metabolites were also found to be related to biosynthetic gene clusters in the genome of HNBCa1. Reblastatin and geldanamycin were uncovered in response to broad-spectrum resistance to the 17 crop pathogenic fungi. Our results suggested that reblastatin and geldanamycin were critical to maintaining the broad-spectrum resistance property and highly efficient antifungal activity of HNBCa1, which could be further developed as a biological control agent to control crop fungal diseases.