Secretory component mediates Candida albicans binding to epithelial cells

Objectives Candida albicans attaches to oral surfaces via a number of mechanisms including adherence mediated by salivary components adsorbed to the C. albicans cell surface. Our goal was to identify the salivary molecules involved. Materials and Methods Biotinylated salivary polypeptides that were...

Full description

Saved in:

Bibliographic Details
Published in:	Oral diseases Vol. 22; no. 1; pp. 69 - 74
Main Authors:	van der Wielen, PA, Holmes, AR, Cannon, RD
Format:	Journal Article
Language:	English
Published:	Denmark Blackwell Publishing Ltd 01-01-2016 Wiley Subscription Services, Inc
Subjects:	adhesion receptor Biotinylation Candida albicans Candida albicans - metabolism Candidiasis, Oral - metabolism Candidiasis, Oral - microbiology Cell Adhesion - physiology Cell Line Dentistry Electrophoresis, Polyacrylamide Gel Epithelial Cells - microbiology Fungal infections Humans immunoglobulin A Immunoglobulin A, Secretory - chemistry Immunoglobulin A, Secretory - metabolism Mouth Mucosa - chemistry Mouth Mucosa - metabolism Mouth Mucosa - microbiology oral candidiasis Oral diseases oral mucosa Peptides - chemistry Platelet Glycoprotein GPIb-IX Complex - chemistry Platelet Glycoprotein GPIb-IX Complex - metabolism saliva Saliva - chemistry Saliva - metabolism Secretory Component - metabolism saliva immunoglobulin A oral mucosa adhesion receptor oral candidiasis
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Objectives Candida albicans attaches to oral surfaces via a number of mechanisms including adherence mediated by salivary components adsorbed to the C. albicans cell surface. Our goal was to identify the salivary molecules involved. Materials and Methods Biotinylated salivary polypeptides that were bound by C. albicans were detected in extracts from washed, saliva‐treated yeast cells by polyacrylamide gel electrophoresis and electroblot or immunoblot transfer analysis and purified by electroelution. Purified material was tested for the ability to promote the adherence of radiolabelled C. albicans yeast cells to cultured epithelial monolayers. Results Three of the polypeptides bound by C. albicans cells were identified as components of secretory IgA, including secretory component. Using non‐denaturing polyacrylamide gel electrophoresis, we demonstrated that secretory component could be detected in its free form in saliva, and was bound by yeast cells. Secretory component which was purified by electroelution from non‐denaturing PAGE‐separated saliva, without detectable complete IgA, promoted adherence of yeast cells to cultured epithelial monolayers in a dose‐dependent fashion. Conclusion These results indicate that despite the inhibitory effect on adherence of IgA specific to C. albicans, IgA components, in particular secretory component, also promote binding to cultured epithelial monolayers.
Bibliography:	ark:/67375/WNG-T4ZQMQVG-R New Zealand Lottery Grants Board istex:500EE62AD61BDBEBD83133917412866E5DD7BBF8 ArticleID:ODI12397 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1354-523X 1601-0825
DOI:	10.1111/odi.12397