Large increase in disulphide bonds containing cytosol proteins after chronic cadmium administration, estimated in isolated rat liver cells

Using histochemical staining, followed by cytophotometric quantitation of disulphide bonds and total protein in isolated liver cells of rats treated for a long time with low doses of CdCl2, a large increase in disulphide bonds containing proteins could be demonstrated in cells of one ploidy class. T...

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Bibliographic Details
Published in:	Histochemistry Vol. 83; no. 3; pp. 227 - 229
Main Authors:	Morselt, A F, van de Hamer, C J, Prinsen, L, Jongstra-Spaapen, E J, Copius Peereboom-Stegeman, J H, Bosch, K S
Format:	Journal Article
Language:	English
Published:	Germany 1985
Subjects:	Animals Cadmium - pharmacology Cytosol - metabolism Disulfides - metabolism Female In Vitro Techniques Kinetics Liver - cytology Liver - drug effects Liver - metabolism Proteins - metabolism Rats Rats, Inbred Strains
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Summary:	Using histochemical staining, followed by cytophotometric quantitation of disulphide bonds and total protein in isolated liver cells of rats treated for a long time with low doses of CdCl2, a large increase in disulphide bonds containing proteins could be demonstrated in cells of one ploidy class. This increase seems to be due to an increase in high molecular weight (HMW) cytosol proteins as estimated biochemically. They probably represent polymers of metallothionein.
ISSN:	0301-5564 1432-119X
DOI:	10.1007/BF00953988