Gene expression pattern and immunoreactive protein localization of LGR7 receptor in human endometrium throughout the menstrual cycle

Relaxin (RLX) is a pregnancy‐associated polypeptide hormone. In non‐pregnant women, the peak of circulating relaxin coincides with the window of endometrial receptivity and both in vivo and in vitro experiments showed that it plays a role in the decidualization process. Recently, two receptors, LGR7...

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Bibliographic Details
Published in:	Molecular human reproduction Vol. 10; no. 2; pp. 85 - 90
Main Authors:	Luna, José Juan, Riesewijk, Anne, Horcajadas, José A., van Os, Roselin de, Domínguez, Francisco, Mosselman, Sietse, Pellicer, Antonio, Simón, Carlos
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 01-02-2004 Oxford Publishing Limited (England)
Subjects:	Biological and medical sciences Cell Line Embryology: invertebrates and vertebrates. Teratology Endometrium - chemistry Endometrium - cytology Endometrium - metabolism Female Fundamental and applied biological sciences. Psychology Gene Expression - genetics Humans Immunochemistry Key words: endometrium/LGR7 receptor/relaxin Luteal Phase - metabolism Membrane Proteins - analysis Membrane Proteins - biosynthesis Membrane Proteins - genetics Membrane Proteins - immunology Menstrual Cycle - metabolism Receptors, G-Protein-Coupled - analysis Receptors, G-Protein-Coupled - biosynthesis Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - immunology Receptors, Peptide Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Human Uterus Menstrual cycle Female genital system Gene expression Localization Endometrium Protein Biological receptor endometrium/LGR7 receptor/relaxin
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Summary:	Relaxin (RLX) is a pregnancy‐associated polypeptide hormone. In non‐pregnant women, the peak of circulating relaxin coincides with the window of endometrial receptivity and both in vivo and in vitro experiments showed that it plays a role in the decidualization process. Recently, two receptors, LGR7 and LGR8, have been identified as high affinity receptors for relaxin. Here we describe LGR7 mRNA and protein expression in human endometrium using semi‐quantitative and quantitative fluorescent PCR (Q‐PCR) and immunohistochemical analyses. Three different experimental designs were used. First, endometrial biopsies from five different phases of the menstrual cycle were analysed. Secondly, we assessed the early luteal phase in more detail. Finally we analysed the expression at LH+2 (2 days after the natural LH surge, pre‐receptive endometrium) versus LH+7 (receptive endometrium) within the same menstrual cycle from the same patient to avoid inter‐cycle or inter‐person variations in gene expression. Our results indicate that there is no consistent regulation of LGR7 mRNA expression, neither during the menstrual cycle nor during the early–mid‐luteal phase. In general, we observed a large degree of variation in LGR7 mRNA expression levels between patients. LGR7 immunoreactive protein was identified in all stages of the menstrual cycle. LGR7 protein was localized in both the epithelial and the stromal compartments, except for the mid‐luteal phase when the expression was restricted to the endometrial epithelium. We conclude that no consistent regulation of LGR7 mRNA expression can be detected in human endometrium during the menstrual cycle.
Bibliography:	5To whom correspondence should be addressed at: Plaza de la Policía Local 3, 46015 Valencia, Spain. e‐mail: csimon@interbook.net istex:9535F72B39252233271EF7371952CCBA6F5EE811 local:gah019 ark:/67375/HXZ-12VJBWV8-3 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1360-9947 1460-2407 1460-2407
DOI:	10.1093/molehr/gah019