Search Results - "van Merkerk, Ronald"

Penicillin V acylases from gram-negative bacteria degrade N-acylhomoserine lactones and attenuate virulence in Pseudomonas aeruginosa by Sunder, Avinash Vellore, Utari, Putri Dwi, Ramasamy, Sureshkumar, van Merkerk, Ronald, Quax, Wim, Pundle, Archana

“…Virulence pathways in gram-negative pathogenic bacteria are regulated by quorum sensing mechanisms, through the production and sensing of N- acylhomoserine…”
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Lipase Expression in Pseudomonas alcaligenes Is Under the Control of a Two-Component Regulatory System by Krzeslak, Joanna, Gerritse, Gijs, van Merkerk, Ronald, Cool, Robbert H, Quax, Wim J

“…Preliminary observations in a large-scale fermentation process suggested that the lipase expression of Pseudomonas alcaligenes can be switched on by the…”
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Development and application of CRISPR‐based genetic tools in Bacillus species and Bacillus phages by Song, Yafeng, He, Siqi, Jopkiewicz, Anita, Setroikromo, Rita, Merkerk, Ronald, Quax, Wim J.

“…Recently, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been developed into a precise and efficient genome editing tool…”
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Tailored photoenzymatic systems for selective reduction of aliphatic and aromatic nitro compounds fueled by light by Luján, Alejandro Prats, Bhat, Mohammad Faizan, Tsaturyan, Sona, van Merkerk, Ronald, Fu, Haigen, Poelarends, Gerrit J.

“…The selective enzymatic reduction of nitroaliphatic and nitroaromatic compounds to aliphatic amines and amino-, azoxy- and azo-aromatics, respectively, remains…”
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Engineering the specificity of Streptococcus pyogenes sortase A by loop grafting by Wójcik, Magdalena, Szala, Kamil, Merkerk, Ronald, Quax, Wim J., Boersma, Ykelien L.

“…Sortases are a group of enzymes displayed on the cell‐wall of Gram‐positive bacteria. They are responsible for the attachment of virulence factors onto the…”
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Metabolic Engineering of Bacillus subtilis Toward Taxadiene Biosynthesis as the First Committed Step for Taxol Production by Abdallah, Ingy I, Pramastya, Hegar, van Merkerk, Ronald, Sukrasno, Quax, Wim J

“…Terpenoids are natural products known for their medicinal and commercial applications. Metabolic engineering of microbial hosts for the production of valuable…”
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Proteolysis Targeting Chimera (PROTAC) for Macrophage Migration Inhibitory Factor (MIF) Has Anti‐Proliferative Activity in Lung Cancer Cells by Xiao, Zhangping, Song, Shanshan, Chen, Deng, Merkerk, Ronald, Wouden, Petra E., Cool, Robbert H., Quax, Wim J., Poelarends, Gerrit J., Melgert, Barbro N., Dekker, Frank J.

“…Macrophage migration inhibitory factor (MIF) is involved in protein‐protein interactions that play key roles in inflammation and cancer. Current strategies to…”
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Creation of RANKL mutants with low affinity for decoy receptor OPG and their potential anti‐fibrosis activity by Wang, Yizhou, Michiels, Timo, Setroikromo, Rita, Merkerk, Ronald, Cool, Robbert H., Quax, Wim J.

“…Fibrosis is characterized by the progressive alteration of the tissue structure due to the excessive production of extracellular matrix (ECM). The signaling…”
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Tuning Enzyme Activity for Nonaqueous Solvents: Engineering an Enantioselective “Michaelase” for Catalysis in High Concentrations of Ethanol by Guo, Chao, Biewenga, Lieuwe, Lubberink, Max, Merkerk, Ronald, Poelarends, Gerrit J.

“…Enzymes have evolved to function under aqueous conditions and may not exhibit features essential for biocatalytic application, such as the ability to function…”
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Catalysis of amorpha-4,11-diene synthase unraveled and improved by mutability landscape guided engineering by Abdallah, Ingy I., van Merkerk, Ronald, Klumpenaar, Esmée, Quax, Wim J.

“…Amorpha-4,11-diene synthase (ADS) cyclizes the substrate farnesyl pyrophosphate to produce amorpha-4,11-diene as a major product. This is considered the first…”
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A regulated synthetic operon facilitates stable overexpression of multigene terpenoid pathway in Bacillus subtilis by Abdallah, Ingy I., Xue, Dan, Pramastya, Hegar, van Merkerk, Ronald, Setroikromo, Rita, Quax, Wim J.

“…The creation of microbial cell factories for sustainable production of natural products is important for medical and industrial applications. This requires…”
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Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases by van der Meer, Jan-Ytzen, Poddar, Harshwardhan, Baas, Bert-Jan, Miao, Yufeng, Rahimi, Mehran, Kunzendorf, Andreas, van Merkerk, Ronald, Tepper, Pieter G., Geertsema, Edzard M., Thunnissen, Andy-Mark W. H., Quax, Wim J., Poelarends, Gerrit J.

“…The Michael-type addition reaction is widely used in organic synthesis for carbon–carbon bond formation. However, biocatalytic methodologies for this type of…”
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PA0305 of Pseudomonas aeruginosa is a quorum quenching acylhomoserine lactone acylase belonging to the Ntn hydrolase superfamily by WAHJUDI, Mariana, PAPAIOANNOU, Evelina, HENDRAWATI, Oktavia, VAN ASSEN, Aart H. G, VAN MERKERK, Ronald, COOL, Robbert H, POELARENDS, Gerrit J, QUAX, Wim J

“…The Pseudomonas aeruginosa PAO1 genome has at least two genes, pvdQ and quiP, encoding acylhomoserine lactone (AHL) acylases. Two additional genes, pa1893 and…”
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Novel RANKL DE‐loop mutants antagonize RANK‐mediated osteoclastogenesis by Wang, Yizhou, Assen, Aart H.G., Reis, Carlos R., Setroikromo, Rita, Merkerk, Ronald, Boersma, Ykelien L., Cool, Robbert H., Quax, Wim J.

“…Bone is a dynamic tissue that is maintained by continuous renewal. An imbalance in bone resorption and bone formation can lead to a range of disorders, such as…”
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LmrCD is a major multidrug resistance transporter in Lactococcus lactis by Lubelski, Jacek, De Jong, Anne, Van Merkerk, Ronald, Agustiandari, Herfita, Kuipers, Oscar P., Kok, Jan, Driessen, Arnold J. M.

“…Summary When Lactococcus lactis is challenged with drugs it displays a multidrug resistance (MDR) phenotype. In silico analysis of the genome of L. lactis…”
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Proteolysis Targeting Chimera (PROTAC) for Macrophage Migration Inhibitory Factor (MIF) Has Anti‐Proliferative Activity in Lung Cancer Cells by Xiao, Zhangping, Song, Shanshan, Chen, Deng, Merkerk, Ronald, Wouden, Petra E., Cool, Robbert H., Quax, Wim J., Poelarends, Gerrit J., Melgert, Barbro N., Dekker, Frank J.

“…Macrophage migration inhibitory factor (MIF) is involved in protein‐protein interactions that play key roles in inflammation and cancer. Current strategies to…”
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Lipase A gene transcription in Pseudomonas alcaligenes is under control of RNA polymerase σ54 and response regulator LipR by Krzeslak, Joanna, Papaioannou, Evelina, van Merkerk, Ronald, Paal, Krisztina A., Bischoff, Rainer, Cool, Robbert H., Quax, Wim J.

“…Abstract Initial analysis has shown that the transcription of the Pseudomonas alcaligenes lipA gene, which encodes an extracellular lipase, is governed by the…”
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ydaG and ydbA of Lactococcus lactis Encode a Heterodimeric ATP-binding Cassette-type Multidrug Transporter by Lubelski, Jacek, Mazurkiewicz, Piotr, van Merkerk, Ronald, Konings, Wil N, Driessen, Arnold J M

“…Multidrug resistance (MDR)-type transporters mediate the active extrusion of structurally and functionally dissimilar compounds from the cells, thereby…”
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Insights into taxadiene synthase catalysis and promiscuity facilitated by mutability landscape and molecular dynamics by He, Siqi, Abdallah, Ingy I., van Merkerk, Ronald, Quax, Wim J.

“…Main conclusion Protein modeling, carbocation docking, and molecular dynamics along with structure-based mutability landscapes provided insight into taxadiene…”
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Production of Squalene in Bacillus subtilis by Squalene Synthase Screening and Metabolic Engineering by Song, Yafeng, Guan, Zheng, van Merkerk, Ronald, Pramastya, Hegar, Abdallah, Ingy I, Setroikromo, Rita, Quax, Wim J

“…Squalene synthase (SQS) catalyzes the conversion of two farnesyl pyrophosphates to squalene, an important intermediate in between isoprene and valuable…”
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