Construction and in vivo evaluation of a dual layered collagenous scaffold with a radial pore structure for repair of the diaphragm

In each organ the extracellular matrix has a specific architecture and composition, adapted to the functional needs of that organ. As cells are known to respond to matrix organization, biomaterials that take into account the specific architecture of the tissues to be regenerated may have an advantag...

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Published in:	Acta biomaterialia Vol. 9; no. 6; pp. 6844 - 6851
Main Authors:	Brouwer, Katrien M., Daamen, Willeke F., van Lochem, Nicole, Reijnen, Daphne, Wijnen, René M.H., van Kuppevelt, Toin H.
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-06-2013
Subjects:	Animals Architecture biocompatible materials Biocompatible Materials - chemical synthesis blood vessels Collagen Collagen - chemistry crosslinking Diaphragm Diaphragm - injuries Diaphragm - pathology Diaphragm - surgery Equipment Failure Analysis extracellular matrix formaldehyde freezing Guided Tissue Regeneration - instrumentation in vivo studies Male medicine muscle fibers Orientation Porosity Prosthesis Design Radial Rats Rats, Wistar Reconstructive Surgical Procedures - instrumentation skeletal muscle Tissue Scaffolds Treatment Outcome vapors Diaphragm Architecture Orientation Radial Collagen
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Summary:	In each organ the extracellular matrix has a specific architecture and composition, adapted to the functional needs of that organ. As cells are known to respond to matrix organization, biomaterials that take into account the specific architecture of the tissues to be regenerated may have an advantage in regenerative medicine. In this study we focussed on the diaphragm, an organ essential for breathing, and consisting of radial oriented skeletal muscle fibres diverging from a central tendon plate. To mimic this structure dual layered collagenous scaffolds were constructed with a radial pore orientation, prepared by inward out freezing, and reinforced by a layer of compressed collagen. Similar scaffolds with a random round pore structure were taken as controls. Scaffolds were first mildly crosslinked by formaldehyde vapour fixation for initial stabilization (13% and 17% crosslinking for the radial and control scaffolds, respectively), and further crosslinked using aqueous 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (38% and 37% crosslinking, respectively). Scaffolds were implanted into a surgically created diaphragm defect in rats and explanted after 12weeks. Macroscopically, integration of the radial scaffolds with the surrounding diaphragm was better compared with the controls. Cells had infiltrated further into the centre of the scaffolds (P=0.029) and there was a tendency of blood vessels to migrate deeper into the radial scaffolds (P=0.057, compared with controls). Elongated cells (SMA-positive) were aligned with the radial structures. In conclusion, collagenous scaffolds with a stable radial pore structure can be constructed which facilitate cellular in-growth and alignment in vivo.
Bibliography:	http://dx.doi.org/10.1016/j.actbio.2013.03.003 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1742-7061 1878-7568
DOI:	10.1016/j.actbio.2013.03.003