Selective and sensitive determination of lactose in low-lactose dairy products with HPAEC-PAD

Selective and sensitive determination of lactose in low-lactose dairy products with HPAEC-PAD

•Determination of low lactose (<0.01%) in wide range of dairy products.•Simple sample preparation steps, i.e. dilution, centrifugation and ultrafiltration.•Superior separation of lactose from other disaccharides by CarboPac PA100 column.•Validated method and participation to international profici...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1060; pp. 395 - 399
Main Authors: van Scheppingen, Wibo B., van Hilten, Piet H., Vijverberg, Marieke P., Duchateau, Alexander L.L.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 15-08-2017
Subjects:
Anion exchange chromatography
Carbohydrates
CarboPac PA100
Chromatography, High Pressure Liquid - methods
Chromatography, Ion Exchange - methods
Dairy Products - analysis
Food products
Lactose - analysis
Lactose free
Limit of Detection
Linear Models
Reproducibility of Results
β-galactosidase
Lactose free
β-galactosidase
Carbohydrates
Food products
CarboPac PA100
Anion exchange chromatography
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Summary:•Determination of low lactose (<0.01%) in wide range of dairy products.•Simple sample preparation steps, i.e. dilution, centrifugation and ultrafiltration.•Superior separation of lactose from other disaccharides by CarboPac PA100 column.•Validated method and participation to international proficiency tests. The demand for low lactose dairy products is increasing and more different lactose free food is commercially available. The level of lactose in these products decreased during the last years and nowadays a concentration of <0.01% is generally accepted as “lactose free”. For the determination of the lactose concentrations in these dairy products a sensitive analysis method is needed. We developed a method for the determination of low concentrations of lactose in a wide range of dairy products. A simple sample preparation with dilution, centrifugation and ultrafiltration is efficient for the isolation of lactose from the sample matrix. In this paper, a new HPAEC-PAD analysis on a CarboPac PA100 column gives a good separation of lactose from the other saccharides. This separation in combination with the PAD detector yields a selective and sensitive method for the quantification at the desired concentrations of lactose in low lactose dairy products.
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ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2017.06.024