Search Results - "van Laar, Theo"

DNA replication origins retain mobile licensing proteins by Sánchez, Humberto, McCluskey, Kaley, van Laar, Theo, van Veen, Edo, Asscher, Filip M., Solano, Belén, Diffley, John F. X., Dekker, Nynke H.

“…DNA replication in eukaryotes initiates at many origins distributed across each chromosome. Origins are bound by the origin recognition complex (ORC), which,…”
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TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the endoplasmic reticulum by Hassink, Gerco, Kikkert, Marjolein, van Voorden, Sjaak, Lee, Shiow-Ju, Spaapen, Robbert, van Laar, Theo, Coleman, Catherine S, Bartee, Eric, Früh, Klaus, Chau, Vincent, Wiertz, Emmanuel

“…In the present study, the human TEB4 is identified as a novel ER (endoplasmic reticulum)-resident ubiquitin ligase. TEB4 has homologues in many species and has…”
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Mutations of the PU.1 Ets domain are specifically associated with murine radiation-induced, but not human therapy-related, acute myeloid leukaemia by SURAWEERA, Nirosha, MEIJNE, Emmy, ROWAN, Andrew, TOMLINSON, Ian P. M, SILVER, Andrew, MOODY, John, CARVAJAL-CARMONA, Luis G, YOSHIDA, Kazuko, POLLARD, Patrick, FITZGIBBON, Jude, RICHES, Andrew, VAN LAAR, Theo, HUISKAMP, Rene

“…Murine radiation-induced acute myeloid leukaemia (AML) is characterized by loss of one copy of chromosome 2. Previously, we positioned the critical…”
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Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation by Ramírez Montero, Daniel, Sánchez, Humberto, van Veen, Edo, van Laar, Theo, Solano, Belén, Diffley, John F. X., Dekker, Nynke H.

“…The eukaryotic replicative helicase CMG centrally orchestrates the replisome and leads the way at the front of replication forks. Understanding the motion of…”
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A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint by Sánchez, Humberto, Liu, Zhaowei, van Veen, Edo, van Laar, Theo, Diffley, John F. X., Dekker, Nynke H.

“…Chromatin replication involves the assembly and activity of the replisome within the nucleosomal landscape. At the core of the replisome is the Mcm2-7 complex…”
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CAF-1 deposits newly synthesized histones during DNA replication using distinct mechanisms on the leading and lagging strands by Rouillon, Clément, Eckhardt, Bruna V, Kollenstart, Leonie, Gruss, Fabian, Verkennis, Alexander E E, Rondeel, Inge, Krijger, Peter H L, Ricci, Giulia, Biran, Alva, van Laar, Theo, Delvaux de Fenffe, Charlotte M, Luppens, Georgiana, Albanese, Pascal, Sato, Koichi, Scheltema, Richard A, de Laat, Wouter, Knipscheer, Puck, Dekker, Nynke H, Groth, Anja, Mattiroli, Francesca

“…Abstract During every cell cycle, both the genome and the associated chromatin must be accurately replicated. Chromatin Assembly Factor-1 (CAF-1) is a key…”
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Invincible DNA tethers: covalent DNA anchoring for enhanced temporal and force stability in magnetic tweezers experiments by Janissen, Richard, Berghuis, Bojk A, Dulin, David, Wink, Max, van Laar, Theo, Dekker, Nynke H

“…Magnetic tweezers are a powerful single-molecule technique that allows real-time quantitative investigation of biomolecular processes under applied force. High…”
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Signatures of Nucleotide Analog Incorporation by an RNA-Dependent RNA Polymerase Revealed Using High-Throughput Magnetic Tweezers by Dulin, David, Arnold, Jamie J., van Laar, Theo, Oh, Hyung-Suk, Lee, Cheri, Perkins, Angela L., Harki, Daniel A., Depken, Martin, Cameron, Craig E., Dekker, Nynke H.

“…RNA viruses pose a threat to public health that is exacerbated by the dearth of antiviral therapeutics. The RNA-dependent RNA polymerase (RdRp) holds promise…”
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Suppression of anoikis and induction of metastasis by the neurotrophic receptor TrkB by Peeper, Daniel S, Douma, Sirith, van Laar, Theo, Zevenhoven, John, Meuwissen, Ralph, van Garderen, Evert

“…Metastasis is a major factor in the malignancy of cancers, and is often responsible for the failure of cancer treatment. Anoikis (apoptosis resulting from loss…”
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Characterizing single-molecule dynamics of viral RNA-dependent RNA polymerases with multiplexed magnetic tweezers by Kuijpers, Louis, van Laar, Theo, Janissen, Richard, Dekker, Nynke H.

“…Multiplexed single-molecule magnetic tweezers (MT) have recently been employed to probe the RNA synthesis dynamics of RNA-dependent RNA polymerases (RdRp)…”
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Strand separation establishes a sustained lock at the Tus–Ter replication fork barrier by Berghuis, Bojk A, Dulin, David, Xu, Zhi-Qiang, van Laar, Theo, Cross, Bronwen, Janissen, Richard, Jergic, Slobodan, Dixon, Nicholas E, Depken, Martin, Dekker, Nynke H

“…Tus protein bound to Ter sites on circular bacterial chromosomes provides a way to avoid random crashes of opposing replication forks. DNA-unzipping…”
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Wnt/β-catenin signal pathway stabilizes APP intracellular domain (AICD) and promotes its transcriptional activity by Zhou, Fangfang, Gong, Kai, Laar, Theo van, Gong, Yandao, Zhang, Long

“…► Wnt activation increases endogenous AICD expression independent of γ-secretase activity. ► Wnt/β-catenin signaling pathway stabilizes AICD. ► β-Catenin…”
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Expression of BNIP3 in invasive breast cancer: correlations with the hypoxic response and clinicopathological features by Koop, Esther A, van Laar, Theo, van Wichen, Dick F, de Weger, Roel A, Wall, Elsken van der, van Diest, Paul J

“…Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 (BNIP3) is a pro-apoptotic member of the Bcl-2 family induced under hypoxia. Low or absent expression has…”
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Essential validation methods for E. coli strains created by chromosome engineering by Tiruvadi Krishnan, Sriram, Moolman, M Charl, van Laar, Theo, Meyer, Anne S, Dekker, Nynke H

“…Chromosome engineering encompasses a collection of homologous recombination-based techniques that are employed to modify the genome of a model organism in a…”
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A role for Rad23 proteins in 26S proteasome-dependent protein degradation? by van Laar, Theo, van der Eb, Alex J, Terleth, Carrol

“…Treatment of cells with genotoxic agents affects protein degradation in both positive and negative ways. Exposure of S. cerevisiae to the alkylating agent MMS…”
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SMC complexes can traverse physical roadblocks bigger than their ring size by Pradhan, Biswajit, Barth, Roman, Kim, Eugene, Davidson, Iain F., Bauer, Benedikt, van Laar, Theo, Yang, Wayne, Ryu, Je-Kyung, van der Torre, Jaco, Peters, Jan-Michael, Dekker, Cees

“…Ring-shaped structural maintenance of chromosomes (SMC) complexes like condensin and cohesin extrude loops of DNA. It remains, however, unclear how they can…”
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The novel MMS-inducible gene Mif1/KIAA0025 is a target of the unfolded protein response pathway by van Laar, Theo, Schouten, Theo, Hoogervorst, Esther, van Eck, Marga, van der Eb, Alex J., Terleth, Carrol

“…In a search for genes induced by DNA-damaging agents, we identified two genes that are activated by methyl methanesulfonate (MMS). Expression of both genes is…”
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Snail and Slug, key regulators of TGF-β-induced EMT, are sufficient for the induction of single-cell invasion by Naber, Hildegonda P.H., Drabsch, Yvette, Snaar-Jagalska, B. Ewa, ten Dijke, Peter, van Laar, Theo

“…•Snail or Slug expression in H-Ras transformed MCF10AT induced an EMT-like phenotype.•Slug or Snail expression in H-Ras transformed MCF10AT promoted invasion…”
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High-throughput, high-force probing of DNA-protein interactions with magnetic tweezers by Berghuis, Bojk A., Köber, Mariana, van Laar, Theo, Dekker, Nynke H.

“…Recent advances in high-throughput single-molecule magnetic tweezers have paved the way for obtaining information on individual molecules as well as…”
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Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG by Ramírez Montero, Daniel, Sánchez, Humberto, van Veen, Edo, van Laar, Theo, Solano, Belén, Diffley, John F X, Dekker, Nynke H

“…Eukaryotes have one replicative helicase known as CMG, which centrally organizes and drives the replisome, and leads the way at the front of replication forks…”
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