Overexpression of Df4CL1 from Dryopteris fragrans Enhances Flavonoids and Lignin Production in Transgenic Tobacco

Overexpression of Df4CL1 from Dryopteris fragrans Enhances Flavonoids and Lignin Production in Transgenic Tobacco

Dryopteris fragrans (L.) Schott is a fern in the family Dryopteridaceae that shows a good curative efficacy against several skin diseases, such as psoriasis and athlete’s foot. One of the substances that are effective is a flavonoid. The enzyme 4-Coumaric acid coenzyme A ligase (4CL) plays a key rol...

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Bibliographic Details
Published in:Russian journal of plant physiology Vol. 68; no. 1; pp. 110 - 117
Main Authors: Li, Sh, Chang, Y., lin Teoh, P., Wang, D., Mo, J., Li, B., Shao, Sh
Format: Journal Article
Language:English
Published: Moscow Pleiades Publishing 2021
Springer Nature B.V
Subjects:
Biomedical and Life Sciences
Biosynthesis
Coenzyme A
Coumaric acid
Dryopteris fragrans
Enzymatic activity
Enzyme activity
Enzymes
Ferns
Flavonoids
Life Sciences
Lignin
Phenylpropanoids
Plant Physiology
Plant Sciences
Proteins
Psoriasis
Relative abundance
Research Papers
Skin diseases
Tobacco
Transgenic plants
CoA ligase enzyme gen
4-coumarate
lignin
flavonoids
phenylpropanoid metabolism function
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Summary:Dryopteris fragrans (L.) Schott is a fern in the family Dryopteridaceae that shows a good curative efficacy against several skin diseases, such as psoriasis and athlete’s foot. One of the substances that are effective is a flavonoid. The enzyme 4-Coumaric acid coenzyme A ligase (4CL) plays a key role in the phenylpropanoid metabolism pathway, which controls the metabolic direction of phenylpropanoids and is associated with the synthesis of flavonoids and lignin. In this paper, we examined the role of 4CL1 gene of D. fragrans ( Df4CL1 ) by transferring it to the model plant tobacco. Then its gene function was analyzed through tissue expression, determination of protein and enzyme activity, and assessment of flavonoids and lignin production. The analysis showed that overexpression of Df4CL1 in the transgenic tobacco (TT) had higher protein content, 4CL activity, and content of flavonoids and lignin compared with wild type tobacco (WT). The tissue expression of Df4CL1 in TT was positively correlated with the content of flavonoids and lignin. Therefore, we conclude that Df4CL1 was associated with both flavonoids and lignin biosynthesis by controlling the relative abundance of flavonoids and lignin during normal development.
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443721010118