Analysis of Genetic Diversity of Fusarium oxysporum f. sp. phaseoli Isolates, Pathogenic and Non-pathogenic to Common Bean (Phaseolus vulgaris L.)

Analysis of Genetic Diversity of Fusarium oxysporum f. sp. phaseoli Isolates, Pathogenic and Non-pathogenic to Common Bean (Phaseolus vulgaris L.)

Twenty isolates of Fusarium oxysporum from Brazil, pathogenic and non-pathogenic to common bean, were analysed using random amplified polymorphic DNA (RAPDs) to study the genetic diversity. RAPD analysis using 23 oligonucleotides resulted in the amplification of 229 polymorphic and 7 monomorphic DNA...

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Bibliographic Details
Published in:Journal of phytopathology Vol. 154; no. 9; pp. 545 - 549
Main Authors: Zanotti, M.G.S, deQueiroz, M.V, dosSantos, J.K, Rocha, R.B, deBarros, E.G, Araújo, E.F
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-09-2006
Blackwell Publishing Ltd
Blackwell
Subjects:
beans
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
fungal diseases of plants
Fungal plant pathogens
Fusarium oxysporum
Fusarium oxysporum f. sp. phaseoli
genetic distance
genetic diversity
genetic variation
molecular markers
pathogenic races
pathogenicity
Phaseolus vulgaris
Phytopathology. Animal pests. Plant and forest protection
plant pathogenic fungi
random amplified polymorphic analysis
strain differences
strains
virulence
Brazil
South America
America
Plant pathology
Genetic variability
Microbiology
Plant pathogen
Genetic distance
Fungi
Dicotyledones
Angiospermae
Fungi Imperfecti
Thallophyta
Data analysis
Fusarium oxysporum
Genetic diversity
random amplified polymorphic analysis
Random amplified polymorphic DNA marker
pathogenic races
Amplification
Pathogenicity
Grain legume
Leguminosae
Vegetable crop
Phaseolus vulgaris
Isolate
Spermatophyta
molecular markers
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Summary:Twenty isolates of Fusarium oxysporum from Brazil, pathogenic and non-pathogenic to common bean, were analysed using random amplified polymorphic DNA (RAPDs) to study the genetic diversity. RAPD analysis using 23 oligonucleotides resulted in the amplification of 229 polymorphic and 7 monomorphic DNA fragments ranging from 234 to 2590 bp. High genetic variability was observed among the isolates, with the distances varying between 8% and 76% among pathogenic, 2% and 63% among the non-pathogenic and 45% and 76% between pathogenic and non-pathogenic isolates. The analysis of genetic distance data showed that the pathogenic isolates tended to group in one group and the non-pathogenic in another. The genetic distance values of 30% among the pathogenic isolates in cluster A are compatible with the genetic distance values observed within the physiological races, but the distance values among the pathogenic isolates in clusters B and G are not compatible with the distance values observed within the race. Although our results are preliminary, it was not possible to exclude the existence of more than one race of this fungus in Brazil.
Bibliography:http://dx.doi.org/10.1111/j.1439-0434.2006.01145.x
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content type line 23
ISSN:0931-1785
1439-0434
DOI:10.1111/j.1439-0434.2006.01145.x