Use of proteomics in the study of the acute phase of caprine arthritis encephalitis in seminal plasma

•The proteomics can be used as complementary diagnosis of CAE.•Clusterin was identified in early seroconversion animals and this protein may be an indicator of the CAEV infection.•The presence of high expression of immunodefense-related proteins is related to animal infection. Caprine arthritis ence...

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Bibliographic Details
Published in:	Small ruminant research Vol. 181; pp. 39 - 44
Main Authors:	dos Santos, Katianne Freitas, Xavier Eloy, Angela Maria, Carneiro Matos, Maria Nágila, Peixoto, Renato Mesquita, de Aragão, Paulo de Tarso Teles Dourado, Rizaldo Pinheiro, Raymundo, da Cunha, Rodrigo Maranguape Silva
Format:	Journal Article
Language:	English
Published:	Elsevier B.V 01-12-2019
Subjects:	Caprine arthritis encephalitis Goat Immunology Proteomics Caprine arthritis encephalitis Goat Immunology Proteomics
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Summary:	•The proteomics can be used as complementary diagnosis of CAE.•Clusterin was identified in early seroconversion animals and this protein may be an indicator of the CAEV infection.•The presence of high expression of immunodefense-related proteins is related to animal infection. Caprine arthritis encephalitis (CAE) is a chronic viral disease spread among dairy goats, caused by the virus of the Lentivirus genus, Orthoretrovirinae subfamily, and Retroviridae family, whose diagnostic tests are ineffective in view of the latency of the virus, making it difficult to control and treat it. As the acute phase of infection has not been studied yet and considering that the innate immune system participates in the initial direct line of defense, during which the virus is transmitted with very low efficiency, the aim of this work was to use proteomics tools to identify proteins and enzymes that may be present at this stage and may serve as indicators of infection. Seminal plasma of 05 Saanen animals was used, before and after infection with the CAE virus, in which two-dimensional electrophoresis was performed, followed by MS mass spectrometry. It was identified in some animals, at the first seroconversion by Western blotting (WB), positive significant expression of the Clusterin (score 789), Zinc-alpha2-glycoprotein (ZAG-2) (score 364), Prostaglandin– H2 D-isomerase precursor (score 85), and Serum Albumin (score 314), exception to Cystatin C (score 58). Taking into account that semen Clusterin in human binds with a very high affinity to DC-SIGN, an important piece in the recognition of pathogens by dendritic cells (DCs), canceling the binding of HIV-1 to DC-SIGN suggesting this protein may be an indicator of the CAEV infection.
ISSN:	0921-4488 1879-0941
DOI:	10.1016/j.smallrumres.2019.10.004