Sex-dependent changes in AMPAR expression and Na, K-ATPase activity in the cerebellum and hippocampus of α-Klotho-Hypomorphic mice

Sex-dependent changes in AMPAR expression and Na, K-ATPase activity in the cerebellum and hippocampus of α-Klotho-Hypomorphic mice

Aging is characterized by a functional decline in several physiological systems. α-Klotho-hypomorphic mice (Kl−/−) exhibit accelerated aging and cognitive decline. We evaluated whether male and female α-Klotho-hypomorphic mice show changes in the expression of synaptic proteins, N-methyl-d-aspartate...

Full description

Saved in:
Bibliographic Details
Published in:Neuropharmacology Vol. 258; p. 110097
Main Authors: dos Santos, Geovana Rosa Oliveira, Cararo-Lopes, Marina Minto, Possebom, Isabela Ribeiro, de Sá Lima, Larissa, Scavone, Cristoforo, Kawamoto, Elisa Mitiko
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-11-2024
Subjects:
Aging
Glutamatergic receptors
K+-ATPase
Na+
Synaptic proteins
α-Klotho
Aging
α-Klotho
Na+
K+-ATPase
Synaptic proteins
Glutamatergic receptors
K(+)-ATPase
glutamatergic receptors
synaptic proteins
aging
Na(+)
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aging is characterized by a functional decline in several physiological systems. α-Klotho-hypomorphic mice (Kl−/−) exhibit accelerated aging and cognitive decline. We evaluated whether male and female α-Klotho-hypomorphic mice show changes in the expression of synaptic proteins, N-methyl-d-aspartate receptor (NMDAR) and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) subunits, postsynaptic density protein 95 (PSD-95), synaptophysin and synapsin, and the activity of Na+, K+-ATPase (NaK) isoforms in the cerebellum and hippocampus. In this study, we demonstrated that in the cerebellum, Kl−/− male mice have reduced expression of GluA1 (AMPA) compared to wild-type (Kl+/+) males and Kl−/− females. Also, Kl−/− male and female mice show reduced ɑ2/ɑ3-NaK and Mg2+-ATPase activities in the cerebellum, respectively, and sex-based differences in NaK and Mg2+-ATPase activities in both the regions. Our findings suggest that α-Klotho could influence the expression of AMPAR and the activity of NaK isoforms in the cerebellum in a sex-dependent manner, and these changes may contribute, in part, to cognitive decline. •Klotho-hypomorphic mice show sex-based reduced AMPAR expression in the cerebellum.•Male Klotho-hypomorphic mice show reduced α2/3-NaK activity in the cerebellum.•Female Klotho-hypomorphic mice show reduced Mg2+-ATPase activity in the cerebellum.•Klotho mice show sex-based distinct NaK activity in the cerebellum and hippocampus.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0028-3908
1873-7064
1873-7064
DOI:10.1016/j.neuropharm.2024.110097