Cytogenotoxic effect, phytochemical screening and antioxidant potential of Jatropha mollissima (Pohl) Baill leaves

Cytogenotoxic effect, phytochemical screening and antioxidant potential of Jatropha mollissima (Pohl) Baill leaves

Phytochemical profile, antioxidant potential, total phenolic content and cytogenotoxic effect (Allium cepa test) were assessed in hexane (HE), ethyl acetate (EA) and methanol (ME) extracts of Jatropha mollissima leaves. Phytochemical analysis revealed the presence of triterpenes in HE, which were al...

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Bibliographic Details
Published in:South African journal of botany Vol. 123; pp. 30 - 35
Main Authors: Dias, W.L.F., do Vale Junior, E.P., das Dores Alves de Oliveira, M., Barbosa, Y.L.P., do Nascimento Silva, J., da Costa Júnior, J.S., de Almeida, P.M., Martins, F.A.
Format: Journal Article
Language:English
Published: Elsevier B.V 01-07-2019
Subjects:
Antimitotic
Antioxidant
Bioassay
Genotoxic
Jatropha mollissima
pinhão-bravo
MN
Bioassay
DPPH
DMSO
TPC
Genotoxic
GAE
Antioxidant
MTT
Jatropha mollissima
MMS
TEAC
Trolox
pinhão-bravo
ME
EC50
EA
MI
Antimitotic
HE
ABTS
CA
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Summary:Phytochemical profile, antioxidant potential, total phenolic content and cytogenotoxic effect (Allium cepa test) were assessed in hexane (HE), ethyl acetate (EA) and methanol (ME) extracts of Jatropha mollissima leaves. Phytochemical analysis revealed the presence of triterpenes in HE, which were also observed in conjunction with flavonoids in EA. Saponins, catechins, phenols, tannins, flavonoids, flavones, flavonols and xanthones were present in ME. The highest antioxidant activity, determined by DPPH free radical scavenging and ABTS assay, and total phenol contents were recorded for ME, followed by EA and HE. All the fractions were cytotoxic in the A. cepa assay, with a significant decline in the mitotic index for HE and EA, whereas proliferative activity in terms of increased mitotic index was observed for ME. ME at concentrations of 1 and 10 mg/mL also caused chromosomal alterations, primarily formation of micronuclei. The study concluded that ME at the lower concentrations (0.01 and 0.1 mg/mL) is promising as an antioxidant, since it did not present a cytogenotoxic effect. HE and EA may be promising in studies of antitumor activity, since all concentrations evaluated inhibited cell division in A. cepa cells and did not present genotoxic activity. •Jatropha mollissima (JM) showed good antioxidant activity in DPPH and ABTS assays.•The methanol fraction of JM exhibited the highest total phenolic content.•The methanol fraction of JM induced cell proliferation in Allium cepa cells.•Hexane and ethyl acetate fractions of JM inhibited cell division in A. cepa cells.
ISSN:0254-6299
1727-9321
DOI:10.1016/j.sajb.2019.02.007