Effects of hyperprolactinemia on the tibial epiphyseal plate of mice treated with sex hormones

The aim of this study was to evaluate the effects of metoclopramide-induced hyperprolactinemia on the tibial epiphyseal plate of hormone-treated oophorectomized mice. For this purpose, 18 animals with intact ovaries were allocated to two groups, M (metoclopramide) and V (vehicle). One hundred and ei...

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Published in:	Gynecological endocrinology Vol. 32; no. 1; pp. 30 - 33
Main Authors:	Wolff, Roberta B., Gomes, Regina Celia T., do Amaral, Vinicius C., da Silva, Priscilla L., Simoncini, Tommaso, Prosdocimi, Fabio Cesar, Simoes, Ricardo S., Simões, Manuel Jesus S., Baracat, Edmund C., Soares-Jr, José Maria
Format:	Journal Article
Language:	English
Published:	England Taylor & Francis 02-01-2016
Subjects:	Androgens - pharmacology Animals Cartilage, Articular - drug effects Cell Proliferation - drug effects Disease Models, Animal Dopamine D2 Receptor Antagonists Estradiol - pharmacology Estrogens Estrogens - pharmacology Female Growth Plate - drug effects hormone replacement therapy Hyperprolactinemia Metoclopramide - pharmacology Mice Osteogenesis - drug effects osteoporosis Ovariectomy Progesterone - pharmacology Progestins - pharmacology Testosterone - pharmacology Tibia - drug effects hormone replacement therapy osteoporosis Estrogens
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Summary:	The aim of this study was to evaluate the effects of metoclopramide-induced hyperprolactinemia on the tibial epiphyseal plate of hormone-treated oophorectomized mice. For this purpose, 18 animals with intact ovaries were allocated to two groups, M (metoclopramide) and V (vehicle). One hundred and eight oophorectomized animals were allocated to 12 subgroups: Oophx/V (vehicle); Ooph/M (metoclopramide); Oophx/V + E (vehicle + estradiol); Oophx/M + E (metoclopramide + estradiol); Oophx/V + P (vehicle + progesterone); Oophx/M + P (metoclopramide + progesterone); Oophx/V + T (vehicle + testosterone); Oophx/M + T (metoclopramide + testosterone); Oophx/V + E + P (Vehicle + estradiol + progesterone); Oophx/M + E + P (metoclopramide + estradiol + progesterone); Oophx/V + E + P + T (vehicle + estradiol + progesterone + testosterone); Oophx/M + E + P + T (metoclopramide + estradiol + progesterone + testosterone). After a 50-day treatment was performed histomorphometric and immunohistochemical cell death analysis. In the epiphyseal plate of the hyperprolactinemic and/or oophorectomized animals, cell proliferation and bone formation decreased, inducing intensified cell death. In the sex steroid-treated animals, estrogen boosted cell proliferation; progesterone, bone formation and testosterone, both cell proliferation and bone formation. These findings suggest that oophorectomy and hyperprolactinemia changed epiphyseal plate morphology causing cartilage degeneration. Treatment with combined sex steroids may diminish such deleterious effects.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0951-3590 1473-0766
DOI:	10.3109/09513590.2015.1068753