An enzyme based biosensor for the direct determination of diisopropyl fluorophosphate

The ability of organophosphate hydrolase (OPH, EC 3.1.8.1) from Pseudomonas diminuta to hydrolyze diisopropyl fluorophosphate (DFP) was used to develop a biosensor for the direct determination of DFP. A fluoride sensitive ion-selective electrode was exploited as the physical transducer for a batch-m...

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Bibliographic Details
Published in:	Analytica chimica acta Vol. 389; no. 1; pp. 189 - 196
Main Authors:	Simonian, A.L, diSioudi, B.D, Wild, J.R
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 14-05-1999 Elsevier
Subjects:	Biological and medical sciences Biosensor Biosensors Biotechnology DFP detection Diisopropyl fluorophosphate Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Organophosphate hydrolase Organophosphorus neurotoxins Various methods and equipments Organophosphorus neurotoxins Diisopropyl fluorophosphate Organophosphate hydrolase DFP detection Biosensor Enzyme Esterases Fluorides Ion selective electrode Phosphoric triester hydrolases Chemical warfare agent Neurotoxin Hydrolases Organophosphorus compounds Aryldialkylphosphatase Dyflos Immobilized enzyme Quantitative analysis
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Summary:	The ability of organophosphate hydrolase (OPH, EC 3.1.8.1) from Pseudomonas diminuta to hydrolyze diisopropyl fluorophosphate (DFP) was used to develop a biosensor for the direct determination of DFP. A fluoride sensitive ion-selective electrode was exploited as the physical transducer for a batch-mode biosensor, and OPH enzyme immobilized on silica gel was used as a biological recognition element. The correlation between DFP concentration and hydrolyzed fluoride extended over a concentration range of 2.5×10 −5–5×10 −3 M. The influence of MeOH on DFP hydrolysis by immobilized OPH was greatly reduced from that of the soluble enzyme, and the effect of paraoxon on DFP detection in mixed samples was investigated.
ISSN:	0003-2670 1873-4324
DOI:	10.1016/S0003-2670(99)00170-1