Lineage analysis of circulating Trypanosoma cruzi parasites and their association with clinical forms of Chagas disease in Bolivia

The causative agent of Chagas disease, Trypanosoma cruzi, is divided into 6 Discrete Typing Units (DTU): Tc I, IIa, IIb, IIc, IId and IIe. In order to assess the relative pathogenicities of different DTUs, blood samples from three different clinical groups of chronic Chagas disease patients (indeter...

Full description

Saved in:

Bibliographic Details
Published in:	PLoS neglected tropical diseases Vol. 4; no. 5; p. e687
Main Authors:	del Puerto, Ramona, Nishizawa, Juan Eiki, Kikuchi, Mihoko, Iihoshi, Naomi, Roca, Yelin, Avilas, Cinthia, Gianella, Alberto, Lora, Javier, Velarde, Freddy Udalrico Gutierrez, Renjel, Luis Alberto, Miura, Sachio, Higo, Hiroo, Komiya, Norihiro, Maemura, Koji, Hirayama, Kenji
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 18-05-2010 Public Library of Science (PLoS)
Subjects:	Adolescent Adult Aged Aged, 80 and over Blood Bolivia Cardiovascular Disorders/Heart Failure Cardiovascular Disorders/Myopathies Chagas Disease - parasitology Chagas Disease - pathology Deoxyribonucleic acid DNA DNA, Kinetoplast - genetics DNA, Protozoan - genetics Female Genotype Humans Immunology/Autoimmunity Infections Infectious Diseases/Epidemiology and Control of Infectious Diseases Infectious Diseases/Neglected Tropical Diseases Male Middle Aged Parasites Pathology Polymerase Chain Reaction Polymorphism, Genetic Studies Tropical diseases Trypanosoma cruzi Trypanosoma cruzi - classification Trypanosoma cruzi - genetics Trypanosoma cruzi - isolation & purification Trypanosoma cruzi - pathogenicity Vector-borne diseases Young Adult Bolivia Latin America South America Central America Humans Middle Aged Genotype Male Bolivia DNA, Protozoan Polymorphism, Genetic Trypanosoma cruzi Chagas Disease DNA, Kinetoplast Young Adult Aged, 80 & over Adolescent Polymerase Chain Reaction Adult Female Aged
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The causative agent of Chagas disease, Trypanosoma cruzi, is divided into 6 Discrete Typing Units (DTU): Tc I, IIa, IIb, IIc, IId and IIe. In order to assess the relative pathogenicities of different DTUs, blood samples from three different clinical groups of chronic Chagas disease patients (indeterminate, cardiac, megacolon) from Bolivia were analyzed for their circulating parasites lineages using minicircle kinetoplast DNA polymorphism. Between 2000 and 2007, patients sent to the Centro Nacional de Enfermedades Tropicales for diagnosis of Chagas from clinics and hospitals in Santa Cruz, Bolivia, were assessed by serology, cardiology and gastro-intestinal examinations. Additionally, patients who underwent colonectomies due to Chagasic magacolon at the Hospital Universitario Japonés were also included. A total of 306 chronic Chagas patients were defined by their clinical types (81 with cardiopathy, 150 without cardiopathy, 100 with megacolon, 144 without megacolon, 164 with cardiopathy or megacolon, 73 indeterminate and 17 cases with both cardiopathy and megacolon). DNA was extracted from 10 ml of peripheral venous blood for PCR analysis. The kinetoplast minicircle DNA (kDNA) was amplified from 196 out of 306 samples (64.1%), of which 104 (53.3%) were Tc IId, 4 (2.0%) Tc I, 7 (3.6%) Tc IIb, 1 (0.5%) Tc IIe, 26 (13.3%) Tc I/IId, 1 (0.5%) Tc I/IIb/IId, 2 (1.0%) Tc IIb/d and 51 (25.9%) were unidentified. Of the 133 Tc IId samples, three different kDNA hypervariable region patterns were detected; Mn (49.6%), TPK like (48.9%) and Bug-like (1.5%). There was no significant association between Tc types and clinical manifestations of disease. None of the identified lineages or sublineages was significantly associated with any particular clinical manifestations in the chronic Chagas patients in Bolivia.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Conceived and designed the experiments: JEN KH. Performed the experiments: RdP KH. Analyzed the data: RdP MK SM HH NK KM KH. Contributed reagents/materials/analysis tools: MK KH. Wrote the paper: RdP KH. Diagnosis of patients Sample collection. Samples storage process: NI. Hemoculture: NI. Sample collection and process (DNA extraction): YR AG JL. Obtained informed consent from patients: CA. Sample collection: CA. Diagnosis of patients (cardiopathy): FUGV. Diagnosis of patients: LAR.
ISSN:	1935-2735 1935-2727 1935-2735
DOI:	10.1371/journal.pntd.0000687