Characterization of the MHC class I-related MR1 locus in nonhuman primates

Characterization of the MHC class I-related MR1 locus in nonhuman primates

We characterized the MHC-related 1 ( MR1) locus in two nonhuman primates species, Pongo pygmaeus and Pan troglodytes. MR1 cDNA sequences encoding several isoforms generated through alternative splicing were observed in both species. Amino acid alignment between the five species in which MR1 has been...

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Bibliographic Details
Published in:Immunogenetics (New York) Vol. 53; no. 8; pp. 643 - 648
Main Authors: Parra Cuadrado, JI, del Moral, MF, Garcia Pavia, P, Setien, F, Martinez Naves, E
Format: Journal Article
Language:English
Published: United States 01-10-2001
Subjects:
Amino Acid Sequence
Animals
Cell Line, Transformed
Cloning, Molecular
Conserved Sequence
Genes, MHC Class I - genetics
Histocompatibility Antigens Class I - genetics
Humans
Minor Histocompatibility Antigens
Molecular Sequence Data
MR1 gene
Pan troglodytes
Pan troglodytes - genetics
Polymerase Chain Reaction
Pongo pygmaeus
Pongo pygmaeus - genetics
Sequence Homology, Amino Acid
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Summary:We characterized the MHC-related 1 ( MR1) locus in two nonhuman primates species, Pongo pygmaeus and Pan troglodytes. MR1 cDNA sequences encoding several isoforms generated through alternative splicing were observed in both species. Amino acid alignment between the five species in which MR1 has been characterized to date - human, chimpanzee, orangutan, mouse, and rat - reveals a very high degree of conservation specially in the alpha1 and alpha2 domains of the molecule. The main differences concentrate in the transmembrane and cytoplasmic domains. In the three primates species there is a lysine residue inside the putative transmembrane domain which is not present in rodents. Furthermore, the MR1 cytoplasmic region is longer in rodents, with a conserved serine-containing motif that could be involved in endocytosis; remarkably, this motif is absent in the three primate species. We also describe the presence in the chimpanzee of a sequence homologous to the MR1P1 pseudogene previously found in humans.
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ISSN:0093-7711
1432-1211
DOI:10.1007/s00251-001-0380-1