Quantitative bioassays for measuring biologically functional gonadotropins based on eel gonadotropic receptors

Highlights ► We developed a bioassay for luteinizing and follicle-stimulating hormone of eels. ► Reporter cells are stably expressing eel gonadotropin receptors and CRE-luc. ► A bioassay protocol was adapted for use of eel blood plasma as a sample. ► This enabled monitoring plasma hormone of hCG inj...

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Bibliographic Details
Published in:	General and comparative endocrinology Vol. 178; no. 1; pp. 145 - 152
Main Authors:	Minegishi, Y, Dirks, R.P, de Wijze, D.L, Brittijn, S.A, Burgerhout, E, Spaink, H.P, van den Thillart, G.E.E.J.M
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-08-2012
Subjects:	adults Anguilla anguilla Anguilla japonica Animals bioassays Biological Assay - methods Blood plasma carp Cell Line Chorionic Gonadotropin - metabolism Eel eel culture Eels - metabolism Endocrinology & Metabolism Follicle Stimulating Hormone - metabolism Follicle-stimulating hormone gonadotropin receptors Gonadotropins - metabolism heat Human chorionic gonadotropin Humans juveniles Luciferase Luteinizing hormone Luteinizing Hormone - metabolism monitoring reporter genes reproduction salmon Salmonidae toxins Japan Luteinizing hormone Follicle-stimulating hormone Eel Human chorionic gonadotropin Luciferase Blood plasma
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Summary:	Highlights ► We developed a bioassay for luteinizing and follicle-stimulating hormone of eels. ► Reporter cells are stably expressing eel gonadotropin receptors and CRE-luc. ► A bioassay protocol was adapted for use of eel blood plasma as a sample. ► This enabled monitoring plasma hormone of hCG injected eels.
Bibliography:	http://dx.doi.org/10.1016/j.ygcen.2012.04.030 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0016-6480 1095-6840
DOI:	10.1016/j.ygcen.2012.04.030