Molecular diversity of Alphacoronavirus 1 in dogs and cats in Colombia

Molecular diversity of Alphacoronavirus 1 in dogs and cats in Colombia

Alphacoronavirus 1 (subgenus Tegacovirus, genus Alphacoronavirus, family Coronaviridae), which encompasses transmissible gastroenteritis virus (TGEV), feline coronavirus (FCoV) and canine coronavirus (CCoV), is an important pathogen that can cause severe gastroenteritis and is distributed worldwide....

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Bibliographic Details
Published in:Heliyon Vol. 6; no. 7; p. e04381
Main Authors: Santana-Clavijo, N.F., Reyes Romero, D.P., Arango Fajardo, D.F., Velandia Muñoz, A., Taniwaki, S.A., de Souza Silva, S.O., Brandão, P.E.
Format: Journal Article
Language:English
Published: Elsevier Ltd 01-07-2020
Elsevier
Subjects:
Alphacoronavirus 1
Biocomputational method
Cats
Colombia
Coronavirus
Dogs
Epidemiology
Genetics
Microbiology
Phylogenetic relationship
Phylogeny
Viral disease
Viral genetics
Virology
Colombia
Alphacoronavirus 1
Cats
Microbiology
Viral genetics
Phylogeny
Epidemiology
Virology
Phylogenetic relationship
Coronavirus
Viral disease
Dogs
Genetics
Biocomputational method
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Summary:Alphacoronavirus 1 (subgenus Tegacovirus, genus Alphacoronavirus, family Coronaviridae), which encompasses transmissible gastroenteritis virus (TGEV), feline coronavirus (FCoV) and canine coronavirus (CCoV), is an important pathogen that can cause severe gastroenteritis and is distributed worldwide. CCoV has two different genotypes: CCoV type I, which has a high identity with FCoV-I, and CCoV type II, which is divided into two subtypes, CCoV IIa (pantropic) and CCoV IIb, which is related to FCoV-II and has been involved in multiple recombination events. Between 2014 and 2018, 43 fecal samples from puppies and young dogs under 1 year of age with hemorrhagic enteritis and from 5 cats under 2 years of age with ascites or thoracic effusion were collected by a private veterinary practice in Bogotá, Colombia. A screening for Coronavirus via RT-PCR (nsp12) and PCR amplification of Canine protoparvovirus (VP1) revealed 27.1% (13/49) and 72.9% (35/49) positive samples, respectively. Positive samples for coronavirus were tested for M, N, S and the sequences grouped in the FCoV, CCoV-I and CCoV-IIb clusters that were distant from the pantropic type (IIa). The N gene formed two clusters, one exclusively with samples from this study in subtype II and another with strains in subtype I. For gene S (subtype I), the samples clustered with the Brazilian samples, while samples positive for S subtype IIb grouped into a cluster distinct from the other reference sequences. The prevalence of coronaviruses identified in this study is within the range reported by different countries worldwide. Genetics; Microbiology; Virology; Biocomputational method; Viral disease; Viral genetics; Phylogeny; Epidemiology; Coronavirus; Alphacoronavirus 1; Dogs; Cats; Colombia; Phylogenetic relationship.
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ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2020.e04381