The toxicological effects of Eryngium foetidum extracts on zebrafish embryos and larvae depend on the type of extract, dose, and exposure time

Abstract Eryngium foetidum is a herbaceous plant found in tropical and subtropical regions. In vivo pharmacological parameters show that leaf extracts of this plant have antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities due to their bioactive compounds such as flavonoids and...

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Bibliographic Details
Published in:	Toxicology research (Cambridge) Vol. 11; no. 5; pp. 891 - 899
Main Authors:	Castro, Tassia Flavia Dias, Carneiro, William Franco, Reichel, Tharyn, Fabem, Sarah Lacerda, Machado, Mônica Rodrigues Ferreira, de Souza, Krisnanda Kelly Castro, Resende, Luciane Vilela, Murgas, Luis David Solis
Format:	Journal Article
Language:	English
Published:	Oxford University Press 29-10-2022
Online Access:	Get full text
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Summary:	Abstract Eryngium foetidum is a herbaceous plant found in tropical and subtropical regions. In vivo pharmacological parameters show that leaf extracts of this plant have antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities due to their bioactive compounds such as flavonoids and phenols. Despite the evidence for several bioactivities of E. foetidum, information on its safety and tolerability is limited. The objective of this study was to assess the effect and concentration of different extracts of E. foetidum on the development of zebrafish (Danio rerio) embryos. To study the impact of aqueous (AE), ethanolic (EE), and methanolic (ME) extracts, the embryos were exposed to 0.625, 1.25, 2.5, 5, and 10 mg mL−1 for up to 120-h postfertilization to assess embryonic developmental toxicity and then to 0.039, 0.078, 0.156, 0.312, and 0.625 mg mL−1 to assess the antioxidant responses of the enzymes superoxide dismutase catalase, glutathione S-transferase (GST), and cell apoptosis. The results showed that, depending on the extraction solvent, concentration used, and exposure time, E. foetidum extracts caused mortality, altered the hatching time, and promoted changes in enzymatic activities. Delays in development and increased GST activity were found in all treatments. Apoptosis was not observed in any of the treatments. In conclusion, AE, EE, and ME concentrations above 0.625 mg mL−1 can cause adverse effects on the early stages of zebrafish development.
ISSN:	2045-4538 2045-452X 2045-4538
DOI:	10.1093/toxres/tfac067