Evaluation of the natural virucidal activity of teas for use in the phage amplification assay

Many natural products have intrinsic antimicrobial activity. In this study we have examined infusions from nine types of loose-leaf tea for their ability to inactivate bacteriophage, for use as an alternative to plant extract in a phage-based Salmonella detection assay. The results demonstrated that...

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Bibliographic Details
Published in:	International journal of food microbiology Vol. 111; no. 3; pp. 259 - 262
Main Authors:	de Siqueira, R.S., Dodd, C.E.R., Rees, C.E.D.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 01-10-2006 Elsevier
Subjects:	antiviral properties Bacteriological Techniques Bacteriophage bacteriophages bioassays Biological and medical sciences Coffee, tea and other stimulative beverage industries Food industries Food Microbiology Fundamental and applied biological sciences. Psychology Humans leaves microbial detection Phage amplification assay plant extracts Plant Extracts - pharmacology rapid methods Salmonella Salmonella Phages - drug effects Salmonella Phages - genetics Salmonella Phages - growth & development Salmonella typhimurium - growth & development Salmonella typhimurium - isolation & purification Salmonella typhimurium - virology Sensitivity and Specificity taxonomy tea Tea - chemistry Time Factors Viral Plaque Assay - methods Virucidal agent Virucidal agent Bacteriophage Phage amplification assay Virus Amplification Evaluation Tea Phage
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Summary:	Many natural products have intrinsic antimicrobial activity. In this study we have examined infusions from nine types of loose-leaf tea for their ability to inactivate bacteriophage, for use as an alternative to plant extract in a phage-based Salmonella detection assay. The results demonstrated that tea infusions, either freshly prepared or stored at 4 °C had virucidal action against two phages, Felix 01 and P22. Crucially, for use in the detection assay, there was no antibacterial effect of the virucide on the target bacteria. Therefore, tea was a good candidate to replace pomegranate as the virucidal agent in the phage amplification assay.
Bibliography:	http://dx.doi.org/10.1016/j.ijfoodmicro.2006.04.047 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0168-1605 1879-3460
DOI:	10.1016/j.ijfoodmicro.2006.04.047