Antimicrobial and Antibiofilm Effect of Brazilian Green Propolis Aqueous Extract against Dental Anaerobic Bacteria

Green propolis may represent a promising therapeutic alternative against dental anaerobic pathogens because of its antimicrobial action. This study aimed to evaluate the antimicrobial and antibiofilm actions of Brazilian green propolis aqueous extract (BGP-AqExt) against dental anaerobic bacteria. T...

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Bibliographic Details
Published in:	Molecules (Basel, Switzerland) Vol. 27; no. 23; p. 8128
Main Authors:	de Sá Assis, Maria Angélica, de Paula Ramos, Lucas, Abu Hasna, Amjad, de Queiroz, Talita Suelen, Pereira, Thaís Cristine, Nagai de Lima, Patrícia Michelle, Berretta, Andresa Aparecida, Marcucci, Maria Cristina, Talge Carvalho, Cláudio Antonio, de Oliveira, Luciane Dias
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 22-11-2022 MDPI
Subjects:	Acids Anaerobic bacteria Analysis Anti-Bacterial Agents - pharmacology Anti-infective agents Anti-Infective Agents - pharmacology Antibiotics Antiinfectives and antibacterials antimicrobial action Antimicrobial agents Bacteria Bacteria, Anaerobic Biofilms Biomass Coumaric acid Dental materials Ethanol Flavonoids Gallic acid green propolis Health aspects Herbal medicine High performance liquid chromatography Hypotheses Infections Kaempferol Liquid chromatography Microbial Sensitivity Tests Microorganisms Minimum inhibitory concentration p-Coumaric acid Pathogens Phenols Porphyromonas gingivalis Propolis Propolis - chemistry Propolis - pharmacology Quercetin Variance analysis Brazil anaerobic bacteria green propolis antimicrobial action
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Summary:	Green propolis may represent a promising therapeutic alternative against dental anaerobic pathogens because of its antimicrobial action. This study aimed to evaluate the antimicrobial and antibiofilm actions of Brazilian green propolis aqueous extract (BGP-AqExt) against dental anaerobic bacteria. The minimum inhibitory concentration (MIC) and minimum microbicide concentration (MMC) of the extract were determined against the standard strains (ATCC) of , , and . BGP-AqExt was chemically characterized by high-performance liquid chromatography with diode-array detection (HPLC-DAD) analysis. Antibiofilm action was measured by MTT and crystal violet tests. The data were statistically analyzed by ANOVA and Tukey (5%) tests. The extract had antimicrobial action against all tested anaerobic bacteria, with an MIC value of 55 mg/mL for all bacteria, an MMC of 27.5 mg/mL for and and 55 mg/mL for . Chemically, BGP-AqExt is composed of quercetin, gallic acid, caffeic and p-coumaric acid, drupani, kaempferol and Artepillin C. Significant reductions in biomass and metabolic action of biofilms were found after BGP-AqExt application. Therefore, BGP-AqExt has an antimicrobial and antibiofilm effect against dental anaerobic bacteria.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1420-3049 1420-3049
DOI:	10.3390/molecules27238128