Detection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotes

Detection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotes

Metalloproteinases (MMPs) are remarkable zinc-dependent endopeptidases, critical for degrading components of the extracellular matrix, thus actively influencing cell migration. Their impact on intracellular parasites, such as the enigmatic protozoan Leishmania, elicits intriguing queries. This study...

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Bibliographic Details
Published in:BMC microbiology Vol. 23; no. 1; pp. 1 - 223
Main Authors: Costa, Brenda Furtado, de Queiroz Filho, Tarcísio Navegante, da Cruz Carneiro, Adeniele Lopes, Falcão, Aline Semblano Carreira, da Silva Kataoka, Maria Sueli, Pinheiro, João de Jesus Viana, Rodrigues, Ana Paula Drummond
Format: Journal Article
Language:English
Published: London BioMed Central Ltd 16-08-2023
BioMed Central
BMC
Subjects:
Analysis
Antibodies
Aprotinin
Care and treatment
Cell migration
Collagen
Collagen matrix
Diagnosis
Dosage and administration
Ethylenediaminetetraacetic acid
Extracellular matrix
Gelatinase A
Gelatinase B
Health aspects
Leishmania
Leishmaniasis
Localization
Matrix metalloproteinases
Metalloproteins
Microscopy
MMP-2
MMP-9
Parasites
Parasitic diseases
Polymerization
Promastigotes
Proteins
Protozoa
Tetracycline
Tetracyclines
Therapeutic applications
Vector-borne diseases
Brazil
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Summary:Metalloproteinases (MMPs) are remarkable zinc-dependent endopeptidases, critical for degrading components of the extracellular matrix, thus actively influencing cell migration. Their impact on intracellular parasites, such as the enigmatic protozoan Leishmania, elicits intriguing queries. This study explores into the untapped territory of MMP-2 and MMP-9 within Leishmania spp. promastigotes. Notably, we successfully detected and quantified these MMPs, while also evaluating their activity in two distinct Leishmania species--L. amazonensis (La) and L. braziliensis (Lb)--at various growth stages and isolated from distinct clinical tegumentar disease forms. The results unveiled the presence of MMP-2 and MMP-9 in both species, albeit with distinct localization patterns. Specifically, MMP-9 exhibited significantly higher gelatinolytic activity in La when compared to Lb. Moreover, our data cleverly illustrated the presence and release of MMP-2 and MMP-9 by La and Lb promastigotes, exposing their ability to invade and migrate within a collagen matrix. This pioneering study establishes a compelling correlation between MMP-2 and MMP-9 and their potential role in the dynamics of La and Lb infection. Suggesting their potential as prognostic markers for severe leishmaniasis and promising target molecules for therapeutic interventions, this research opens new avenues for combatting this debilitating parasitic disease.
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ISSN:1471-2180
1471-2180
DOI:10.1186/s12866-023-02973-z