On the use of 2,1,3-benzothiadiazole derivatives as selective live cell fluorescence imaging probes

Newly designed 2,1,3-benzothiadiazole-containing fluorescent probes with four excited state intramolecular proton transfer (ESIPT) sites were successfully tested in live cell-imaging assays using a confluent monolayer of human stem-cells (tissue). All tested dyes were compared with the commercially...

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Bibliographic Details
Published in:	Bioorganic & medicinal chemistry letters Vol. 20; no. 20; pp. 6001 - 6007
Main Authors:	Oliveira, Felipe F.D., Santos, Diego C.B.D., Lapis, Alexandre A.M., Corrêa, José R., Gomes, Alexandre F., Gozzo, Fabio C., Moreira, Paulo F., de Oliveira, Virgínia C., Quina, Frank H., Neto, Brenno A.D.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Ltd 15-10-2010 Elsevier
Subjects:	Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Benzothiadiazole Biological and medical sciences Cell Survival Cells, Cultured DNA, Single-Stranded - analysis Dyes ESIPT Fluorescent Dyes - analysis Fluorescent Dyes - chemistry Fundamental and applied biological sciences. Psychology Humans Live cell-imaging Spectrometry, Fluorescence Stem Cells - cytology Stem-cells Synthesis Thiadiazoles - analysis Thiadiazoles - chemistry Live cell-imaging Stem-cells Benzothiadiazole Synthesis ESIPT Human Sulfur nitrogen heterocycle Visual imagery Stem cell Selectivity Fluorescence spectrometry Monolayer culture Fluorophore Bicyclic compound Chemical synthesis
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Summary:	Newly designed 2,1,3-benzothiadiazole-containing fluorescent probes with four excited state intramolecular proton transfer (ESIPT) sites were successfully tested in live cell-imaging assays using a confluent monolayer of human stem-cells (tissue). All tested dyes were compared with the commercially available DAPI and gave far better results.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0960-894X 1464-3405
DOI:	10.1016/j.bmcl.2010.08.073