Effects of hexestrol on mouse ovarian morphology and ovulation

Abstract Objective To analyze histological aspects of ovaries as well as the ovulation of adult mice treated with the anabolic agent hexestrol. Methods Thirty adult mice were divided into three groups of 10 animals each: (GI) the animals received a dose of 3 mg/kg of hexestrol; (GII) the animals wer...

Full description

Saved in:

Bibliographic Details
Published in:	Maturitas Vol. 60; no. 2; pp. 153 - 157
Main Authors:	de Oliveira, Joaquim Moraes, Simões, Manuel J, Mora, Oswaldo Alves, Simões, Ricardo Santos, Oliveira-Filho, Ricardo M, Oliveira, Patrícia B, Baracat, Edmund C, Soares, José Maria
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier Ireland Ltd 20-06-2008 Elsevier Science
Subjects:	Anabolic Agents - pharmacology Animals Biological and medical sciences Corpus Luteum - drug effects Corpus Luteum - pathology Drug toxicity and drugs side effects treatment Female Hexestrol Hexestrol - pharmacology Hormones. Endocrine system Internal Medicine Medical sciences Mice Morphology Obstetrics and Gynecology Ovarian Follicle - drug effects Ovarian Follicle - pathology Ovary Ovulation - drug effects Pharmacology. Drug treatments Toxicity: urogenital system Ovary Hexestrol Mice Morphology Toxicity Estrogen Rodentia Ovulation Vertebrata Mammalia Mouse Fertility Animal Anabolic agent Mechanism of action
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Abstract Objective To analyze histological aspects of ovaries as well as the ovulation of adult mice treated with the anabolic agent hexestrol. Methods Thirty adult mice were divided into three groups of 10 animals each: (GI) the animals received a dose of 3 mg/kg of hexestrol; (GII) the animals were given a dose of 6 mg/kg of hexestrol; (GIII) the animals were injected with distilled water (vehicle). Another 10-animal group (GIV) was included, and these mice were injected with propionate testosterone (1.25 mg) after 5 days from the day of birth. Hexestrol was administered intraperitoneally once a day and the treatment lasted 30 days. The mice were then sacrificed; their ovaries and oviducts were removed, submitted to histological routine and analyzed under light microscopy. Results In mice treated with hexestrol (6 mg/kg) (Group II), ovaries were smaller than those from the controls but highly vascularized; similar results were obtained in GIV. A great number of follicles in several stages of development were found – however, with no corpora lutea – in six animals in GII. No corpora lutea were found in GIV. The number of luteal bodies and oocytes in GII was lower than that in GI or GIII. No oocytes were detected in GIV. Finally, the nuclear volume of interstitial cells in GII and GIV was the largest. Conclusion Our data suggest that the anabolic agent hexestrol in a high dose may decrease ovulation in mice.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0378-5122 1873-4111
DOI:	10.1016/j.maturitas.2008.05.002