Profile of Enterobacteria Resistant to Beta-Lactams

Profile of Enterobacteria Resistant to Beta-Lactams

A serious emerging problem worldwide is increased antimicrobial resistance. Acquisition of coding genes for evasion methods of antimicrobial drug mechanisms characterizes acquired resistance. This phenomenon has been observed in Enterobacteriaceae family. Treatment for bacterial infections is perfor...

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Published in:Antibiotics (Basel) Vol. 9; no. 7; p. 410
Main Authors: Santos, Andressa Liberal, dos Santos, Adailton Pereira, Ito, Célia Regina Malveste, Queiroz, Pedro Henrique Pereira de, de Almeida, Juliana Afonso, de Carvalho Júnior, Marcos Antonio Batista, de Oliveira, Camila Zanatta, Avelino, Melissa Ameloti G., Wastowski, Isabela Jubé, Gomes, Giselle Pinheiro Lima Aires, Souza, Adenícia Custódia Silva e, Vasconcelos, Lara Stefânia Netto de Oliveira Leão, Santos, Mônica de Oliveira, da Silva, Carla Afonso, Carneiro, Lilian Carla
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-07-2020
MDPI
Subjects:
Amoxicillin
Ampicillin
Annual reports
antibiogram
Antibiotics
Antimicrobial agents
Antimicrobial resistance
Bacteria
Bacterial diseases
Bacterial infections
Coding
Correlation analysis
Drug resistance
Enterobacter
Enterobacteriaceae
enterobacterias
Enzymes
Genes
Gram-negative bacteria
Health aspects
Identification methods
molecular diagnosis
Nosocomial infections
Public health
Taxonomy
β Lactamase
β-Lactam antibiotics
β-lactams
France
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Summary:A serious emerging problem worldwide is increased antimicrobial resistance. Acquisition of coding genes for evasion methods of antimicrobial drug mechanisms characterizes acquired resistance. This phenomenon has been observed in Enterobacteriaceae family. Treatment for bacterial infections is performed with antibiotics, of which the most used are beta-lactams. The aim of this study was to correlate antimicrobial resistance profiles in Enterobacteriaceae by phenotypic methods and molecular identification of 14 beta-lactamase coding genes. In this study, 70 exclusive isolates from Brazil were used, half of which were collected in veterinary clinics or hospitals Phenotypic methodologies were used and real-time PCR was the molecular methodology used, through the Sybr Green system. Regargding the results found in the tests it was observed that 74.28% were resistant to ampicillin, 62.85% were resistant to amoxicillin associated with clavalunate. The mechanism of resistance that presented the highest expression was ESBL (17.14%). The genes studied that were detected in a greater number of species were blaGIM and blaSIM (66.66% of the samples) and the one that was amplified in a smaller number of samples was blaVIM (16.66%). Therefore, high and worrying levels of antimicrobial resistance have been found in enterobacteria, and a way to minimize the accelerated emergence of their resistance includes developing or improving techniques that generate diagnoses with high efficiency and speed.
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ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics9070410