The Expression of Lipoprotein Receptors Is Increased in the Infarcted Area After Myocardial Infarction Induced in Rats With Cardiac Dysfunction

The Expression of Lipoprotein Receptors Is Increased in the Infarcted Area After Myocardial Infarction Induced in Rats With Cardiac Dysfunction

Left ventricular (LV) remodeling after myocardial infarction constitutes the structural basis for ventricular dysfunction and heart failure. The characterization underlying the expression of lipoprotein receptors in cardiac dysfunction is scarcely explored. The aim of this study was to analyze the s...

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Published in:Lipids Vol. 53; no. 2; pp. 177 - 187
Main Authors: de Lima, Aline D., Guido, Maria C., Tavares, Elaine R., Carvalho, Priscila O., Marques, Alyne F., de Melo, Marcelo D. T., Salemi, Vera M. C., Kalil‐Filho, Roberto, Maranhão, Raul C.
Format: Journal Article
Language:English
Published: Hoboken, USA John Wiley & Sons, Inc 01-02-2018
Subjects:
Animals
Coronary artery disease
Gene Expression Profiling
Heart lipid metabolism
Ketamine
Lipoprotein receptors
Male
Myocardial Infarction - chemically induced
Myocardial Infarction - genetics
Myocardial Infarction - metabolism
Rats
Rats, Wistar
Receptors, Lipoprotein - genetics
Receptors, Lipoprotein - metabolism
Ventricular Dysfunction, Left - chemically induced
Ventricular Dysfunction, Left - genetics
Ventricular Dysfunction, Left - metabolism
Xylazine
Heart lipid metabolism
Lipoprotein receptors
Coronary artery disease
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Summary:Left ventricular (LV) remodeling after myocardial infarction constitutes the structural basis for ventricular dysfunction and heart failure. The characterization underlying the expression of lipoprotein receptors in cardiac dysfunction is scarcely explored. The aim of this study was to analyze the status of lipoprotein receptors on the infarcted and noninfarcted areas of LV and to verify whether nanoparticles that mimic the lipid structure of low‐density lipoprotein (LDL) and have the ability to bind to LDL receptors (LDE) are taken up more avidly by the noninfarcted LV. 13 male Wistar rats with left coronary artery ligation (myocardial infarction [MI]) and 12 animals with SHAM operation (SHAM) were used in this study. 6 weeks after the procedure, the quantification of low‐density lipoprotein receptor (LDLR), LDL receptor‐related protein 1 (LRP1), scavenger receptor‐class B type I (SR‐BI) lipoprotein receptors, and PCNA proliferation marker, and tissue uptake of radioactively labeled LDE were performed. Immunohistochemistry and Western blot analysis showed that LDLR, LRP1, SR‐BI, and PCNA, expression in infarcted area of MI was remarkably higher than SHAM and noninfarcted subendocardial (SEN) and interstitial (INT) areas. In addition, in SEN noninfarcted area of MI, the presence of LDLR was about threefold higher than in SHAM SEN and INT noninfarcted areas. The LDE uptake of noninfarcted LV of MI group was about 30% greater than that of SHAM group. In conclusion, these findings regarding the status of lipoprotein receptors after MI induction could help to establish mechanisms on myocardial repairing. In conclusion, infarcted rats with LV dysfunction showed increased expression of lipoprotein receptors mainly in the infarcted area.
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ISSN:0024-4201
1558-9307
DOI:10.1002/lipd.12014