1274 full-open reading frames of transcripts expressed in the developing mouse nervous system

As part of the trans-National Institutes of Health (NIH) Mouse Brain Molecular Anatomy Project (BMAP), and in close coordination with the NIH Mammalian Gene Collection Program (MGC), we initiated a large-scale project to clone, identify, and sequence the complete open reading frame (ORF) of transcri...

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Published in:	Genome research Vol. 14; no. 10B; pp. 2053 - 2063
Main Authors:	Bonaldo, Maria F, Bair, Thomas B, Scheetz, Todd E, Snir, Einat, Akabogu, Ike, Bair, Jennifer L, Berger, Brian, Crouch, Keith, Davis, Aja, Eyestone, Mari E, Keppel, Catherine, Kucaba, Tamara A, Lebeck, Mark, Lin, Jenny L, de Melo, Anna I R, Rehmann, Joshua, Reiter, Rebecca S, Schaefer, Kelly, Smith, Christina, Tack, Dylan, Trout, Kurtis, Sheffield, Val C, Lin, Jim J-C, Casavant, Thomas L, Soares, Marcelo B
Format:	Journal Article
Language:	English
Published:	United States Cold Spring Harbor Laboratory Press 01-10-2004
Subjects:	Animals Brain - anatomy & histology Brain - embryology Brain - metabolism DNA, Complementary Eye - anatomy & histology Eye - embryology Eye - metabolism Female Gene Expression Profiling Gene Expression Regulation, Developmental - physiology Gene Library Genome Letters Mice Mice, Inbred C57BL Nerve Tissue Proteins - classification Nerve Tissue Proteins - genetics Open Reading Frames - genetics Pregnancy RNA, Messenger - genetics RNA, Messenger - metabolism
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Summary:	As part of the trans-National Institutes of Health (NIH) Mouse Brain Molecular Anatomy Project (BMAP), and in close coordination with the NIH Mammalian Gene Collection Program (MGC), we initiated a large-scale project to clone, identify, and sequence the complete open reading frame (ORF) of transcripts expressed in the developing mouse nervous system. Here we report the analysis of the ORF sequence of 1274 cDNAs, obtained from 47 full-length-enriched cDNA libraries, constructed by using a novel approach, herein described. cDNA libraries were derived from size-fractionated cytoplasmic mRNA isolated from brain and eye tissues obtained at several embryonic stages and postnatal days. Altogether, including the full-ORF MGC sequences derived from these libraries by the MGC sequencing team, NIH_BMAP full-ORF sequences correspond to approximately 20% of all transcripts currently represented in mouse MGC. We show that NIH_BMAP clones comprise 68% of mouse MGC cDNAs > or =5 kb, and 54% of those > or =4 kb, as of March 15, 2004. Importantly, we identified transcripts, among the 1274 full-ORF sequences, that are exclusively or predominantly expressed in brain and eye tissues, many of which encode yet uncharacterized proteins.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Corresponding author. E-MAIL bento-soares@uiowa.edu ; FAX (319) 335-9565.
ISSN:	1088-9051 1549-5469
DOI:	10.1101/gr.2601304