Neuromuscular action of venom from the South American colubrid snake Philodryas patagoniensis

Snakes of the opisthoglyphous genus Philodryas are widespread in South America and cause most bites by colubrids in this region. In this study, we examined the neurotoxic and myotoxic effects of venom from Philodryas patagoniensis in biventer cervicis and phrenic nerve–diaphragm preparations and we...

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Published in:	Comparative biochemistry and physiology. Toxicology & pharmacology Vol. 148; no. 1; pp. 31 - 38
Main Authors:	Carreiro da Costa, Roberta S., Prudêncio, Luiz, Ferrari, Erika Fonseca, Souza, Gustavo H.M.F., de Mello, Sueli Moreira, Prianti Júnior, Antonio Carlos Guimarães, Ribeiro, Wellington, Zamunér, Stella Regina, Hyslop, Stephen, Cogo, José Carlos
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-07-2008
Subjects:	Animals Antivenins - metabolism Antivenom Bothrops Bothrops jararaca Chickens Chromatography, High Pressure Liquid Colubrid Colubridae - metabolism Cross Reactions Crotalid Venoms - metabolism Diaphragm - drug effects Diaphragm - innervation Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel ELISA Enzyme-Linked Immunosorbent Assay Esterases - analysis In Vitro Techniques Male Mice Molecular Weight Muscle Contraction - drug effects Necrosis Neuromuscular Blocking Agents - metabolism Neuromuscular Blocking Agents - pharmacology Neuromuscular effects Neuromuscular Junction - drug effects Neuromuscular Junction - pathology Peptide Hydrolases - analysis Philodryas olfersii Philodryas patagoniensis Phospholipases A2 - analysis Phrenic Nerve - drug effects Snake Venoms - enzymology Snake Venoms - metabolism Snake Venoms - pharmacology South America Time Factors South America Philodryas patagoniensis Antivenom Colubrid Neuromuscular effects ELISA
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Summary:	Snakes of the opisthoglyphous genus Philodryas are widespread in South America and cause most bites by colubrids in this region. In this study, we examined the neurotoxic and myotoxic effects of venom from Philodryas patagoniensis in biventer cervicis and phrenic nerve–diaphragm preparations and we compared the biochemical activities of venoms from P. patagoniensis and Philodryas olfersii. Philodryas patagoniensis venom (40 μg/mL) had no effect on mouse phrenic nerve–diaphragm preparations but caused time-dependent neuromuscular blockade of chick biventer cervicis preparations. This blockade was not reversed by washing. The highest concentration of venom tested (40 μg/mL) significantly reduced ( p < 0.05) the contractures to exogenous acetylcholine (55 μM and 110 μM) and K + (13.4 mM) after 120 min; lower concentrations of venom had no consistent or significant effect on these responses. Venom caused a concentration- and time-dependent release of creatine kinase (CK) from biventer cervicis preparations. Histological analysis showed contracted muscle fibers at low venom concentrations and myonecrosis at high concentrations. Philodryas venoms had low esterase and phospholipase A 2 but high proteolytic activities compared to the pitviper Bothrops jararaca. SDS-PAGE showed that the Philodryas venoms had similar electrophoretic profiles, with most proteins having a molecular mass of 25–80 kDa. Both of the Philodryas venoms cross-reacted with bothropic antivenom in ELISA, indicating the presence of proteins immunologically related to Bothrops venoms. RP-HPLC of P. patagoniensis venom yielded four major peaks, each of which contained several proteins, as shown by SDS-PAGE. These results indicate that P. patagoniensis venom has neurotoxic and myotoxic components that may contribute to the effects of envenoming by this species.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1532-0456 1878-1659
DOI:	10.1016/j.cbpc.2008.03.006