Oncotic colpocytology stained with Harris-Shorr in the observation of vaginal microorganisms

The purpose of this work was to evaluate the efficacy of oncotic colpocytology stained with Harris–Shorr in the identification of the cervicovaginal microflora and infectious agents. Results of microbiologic evaluation carried out in colpocytology exams, bacterioscopy (Gram), and direct exams of 2,0...

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Published in:	Diagnostic cytopathology Vol. 36; no. 6; pp. 358 - 362
Main Authors:	Storti-Filho, Agenor, Estivalet Svidizinski, Terezinha Inez, da Silva Souza, Robson José, de Mello, Ione Cristina Jorge, da Costa Souza, Paola, Lopes Consolaro, Marcia Edilaine
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-06-2008
Subjects:	Adolescent Adult Aged Aged, 80 and over Animals Candidiasis, Vulvovaginal - diagnosis Candidiasis, Vulvovaginal - microbiology cervicovaginal infections Female Gentian Violet Harris-Shorr staining Humans Lactobacillus - isolation & purification Middle Aged oncotic colpocytology Phenazines Sensitivity and Specificity Staining and Labeling Trichomonas vaginalis - isolation & purification Trichomonas Vaginitis - diagnosis Trichomonas Vaginitis - microbiology Vagina - microbiology vaginal microflora Vaginal Smears Vaginosis, Bacterial - diagnosis Vaginosis, Bacterial - microbiology
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Summary:	The purpose of this work was to evaluate the efficacy of oncotic colpocytology stained with Harris–Shorr in the identification of the cervicovaginal microflora and infectious agents. Results of microbiologic evaluation carried out in colpocytology exams, bacterioscopy (Gram), and direct exams of 2,017 women aged from 13 to 80 years were compared. Of these, 83.1% agreed between cytology and Gram, 3.6% partially agreed, and 12.8% disagreed. The predominant microflora was of lactobacilli (63.3%), followed by mixed flora (32.1%). The results of sensitivity to lactobacilli were 96.1% and to mixed flora 88.0%; the specificity values were 91.2 and 92.0%, respectively. Colpocytology detected all the instances of trichomoniasis observed at direct exam (0.6%). The most frequent infectious agents were of candidiasis (14.8%, sensitivity 80.3%) and bacterial vaginosis (11.9%, sensitivity 68.1%). Thus, Harris–Shorr stained cytology was shown to be an excellent diagnostic method for T. vaginalis, lactobacilli, mixed flora, and candidiasis. Diagn. Cytopathol. 2008;36:358–362. © 2008 Wiley‐Liss, Inc.
Bibliography:	ArticleID:DC20820 Souza Laboratory of Pathology, Maringá, Paraná, Brazil ark:/67375/WNG-TGH8DHB5-8 istex:525213996C041C2D47E9EA9A2A091373E697C9CA ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	8755-1039 1097-0339
DOI:	10.1002/dc.20820