Conditioned Media from Human Pulp Stem Cell Cultures Improve Bone Regeneration in Rat Calvarial Critical-Size Defects

Conditioned Media from Human Pulp Stem Cell Cultures Improve Bone Regeneration in Rat Calvarial Critical-Size Defects

The aim of this study was to test whether lyophilized conditioned media from human dental pulp mesenchymal stem cell cultures promote the healing of critical-size defects created in the calvaria of rats. Prior to the surgical procedure, the medium in which dental pulp stem cells were cultured was fr...

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Bibliographic Details
Published in:Journal of functional biomaterials Vol. 14; no. 8; p. 396
Main Authors: Buss, Leonardo Fernandes, de Martin, Gustavo Sigrist, Martinez, Elizabeth Ferreira, Filgueiras, Isabela Amanda de Abreu Araújo Porcaro, Magnabosco, José Luiz, Alves, Bruno Frenhan, de Macedo Almeida, Bruno, Kotaka, Tatiana, Teixeira, Marcelo Lucchesi, Ferreira, José Ricardo Muniz, Rocha, Daniel Navarro da, Canal, Raul, Aloise, Antonio Carlos, Holliday, Lexie Shannon, Pelegrine, André Antonio
Format: Journal Article
Language:English
Published: Basel MDPI AG 25-07-2023
MDPI
Subjects:
Anesthesia
Angiogenesis
Animals
Antibodies
Biomedical materials
Blood vessels
Bone growth
bone regeneration
Calvaria
CD34 antigen
Cell culture
Collagen
conditioned media
Defects
Dental pulp
Growth factors
Mesenchymal stem cells
Osteogenesis
Regeneration
Regeneration (physiology)
Rifamycins
Stem cell research
Stem cells
Vascular endothelial growth factor
Vascularization
Xenografts
Xenotransplantation
Brazil
Germany
St Louis Missouri
United States > US
Switzerland
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Summary:The aim of this study was to test whether lyophilized conditioned media from human dental pulp mesenchymal stem cell cultures promote the healing of critical-size defects created in the calvaria of rats. Prior to the surgical procedure, the medium in which dental pulp stem cells were cultured was frozen and lyophilized. After general anesthesia, an 8 mm diameter bone defect was created in the calvaria of twenty-four rats. The defects were filled with the following materials: xenograft alone (G1) or xenograft associated with lyophilized conditioned medium (G2). After 14 or 42 days, the animals were euthanized, and the specimens processed for histologic and immunohistochemical analysis. Bone formation at the center of the defect was observed only in the G2 at 42 days. At both timepoints, increased staining for VEGF, a marker for angiogenesis, was observed in G2. Consistent with this, at 14 days, G2 also had a higher number of blood vessels detected by immunostaining with an anti-CD34 antibody. In conclusion, conditioned media from human dental pulp mesenchymal stem cell cultures had a positive effect on the regenerative process in rat critical-size bone defects. Both the formation of bone and enhancement of vascularization were stimulated by the conditioned media.
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ISSN:2079-4983
2079-4983
DOI:10.3390/jfb14080396