Search Results - "de Maagd, Ruud A."
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Re-evaluation of transcription factor function in tomato fruit development and ripening with CRISPR/Cas9-mutagenesis
Published in Scientific reports (08-02-2019)“…Tomato ( Solanum lycopersicum ) is a model for climacteric fleshy fruit ripening studies. Tomato ripening is regulated by multiple transcription factors…”
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High-throughput sgRNA testing reveals rules for Cas9 specificity and DNA repair in tomato cells
Published in Frontiers in genome editing (06-06-2023)“…CRISPR/Cas9 technology has the potential to significantly enhance plant breeding. To determine the specificity and the mutagenic spectrum of SpCas9 in tomato,…”
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Revisiting the Role of Master Regulators in Tomato Ripening
Published in Trends in plant science (01-03-2020)“…The study of transcriptional regulation of tomato ripening has been led by spontaneous mutations in transcription factor (TF) genes that completely inhibit…”
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Constitutive Activation of the Midgut Response to Bacillus thuringiensis in Bt-Resistant Spodoptera exigua
Published in PloS one (17-09-2010)“…Bacillus thuringiensis is the most effective microbial control agent for controlling numerous species from different insect orders. The main threat for the…”
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Transcriptional control of fleshy fruit development and ripening
Published in Journal of experimental botany (01-08-2014)“…Growth and ripening of fleshy fruits are intricately regulated and yield an important part of the human diet. Here we review current knowledge of the…”
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Identification of microRNA targets in tomato fruit development using high-throughput sequencing and degradome analysis
Published in Journal of experimental botany (01-04-2013)“…MicroRNAs (miRNAs) play important roles in plant development through regulation of gene expression by mRNA degradation or translational inhibition. Despite the…”
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The Tomato FRUITFULL Homologs TDR4/FUL1 and MBP7/FUL2 Regulate Ethylene-Independent Aspects of Fruit Ripening
Published in The Plant cell (01-11-2012)“…Tomato (Solanum lycopersicum) contains two close homologs of the Arabidopsis thaliana MADS domain transcription factor FRUITFULL (FUL), FUL1 (previously called…”
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Comparison of Cas12a and Cas9-mediated mutagenesis in tomato cells
Published in Scientific reports (24-02-2024)“…Cas12a is a promising addition to the CRISPR toolbox, offering versatility due to its TTTV-protospacer adjacent motif (PAM) and the fact that it induces…”
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Mutations in the Bacillus thuringiensis Cry1Ca toxin demonstrate the role of domains II and III in specificity towards Spodoptera exigua larvae
Published in Biochemical journal (15-12-2004)“…Several mutants of the Bacillus thuringiensis Cry1Ca toxin affected with regard to specific activity towards Spodoptera exigua were studied. Alanine was used…”
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FLOWERING LOCUS C in monocots and the tandem origin of angiosperm-specific MADS-box genes
Published in Nature communications (2013)“…MADS-domain transcription factors have been shown to act as key repressors or activators of the transition to flowering and as master regulators of…”
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The tomato CAROTENOID CLEAVAGE DIOXYGENASE8 (SLCCD8) regulates rhizosphere signaling, plant architecture and affects reproductive development through strigolactone biosynthesis
Published in The New phytologist (01-10-2012)“…Strigolactones are plant hormones that regulate both above-and belowground plant architecture. Strigolactones were initially identified as rhizosphere…”
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Occurrence and Nature of Off-Target Modifications by CRISPR-Cas Genome Editing in Plants
Published in ACS agricultural science & technology (18-04-2022)“…CRISPR-Cas-based genome editing allows for precise and targeted genetic modification of plants. Nevertheless, unintended off-target edits can arise that might…”
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Regulation of tomato fruit pericarp development by an interplay between CDKB and CDKA1 cell cycle genes
Published in Journal of experimental botany (01-04-2012)“…Growth of tomato fruits is determined by cell division and cell expansion, which are tightly controlled by factors that drive the core cell cycle. The…”
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Identification of Loci Affecting Accumulation of Secondary Metabolites in Tomato Fruit of a Solanum lycopersicum × Solanum chmielewskii Introgression Line Population
Published in Frontiers in plant science (28-09-2016)“…Semi-polar metabolites such as flavonoids, phenolic acids, and alkaloids are very important health-related compounds in tomato. As a first step to identify…”
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STRUCTURE, DIVERSITY, AND EVOLUTION OF PROTEIN TOXINS FROM SPORE-FORMING ENTOMOPATHOGENIC BACTERIA
Published in Annual review of genetics (01-01-2003)“…Gram-positive spore-forming entomopathogenic bacteria can utilize a large variety of protein toxins to help them invade, infect, and finally kill their hosts,…”
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Bacillus thuringiensis Cry1Ca-resistant Spodoptera exigua lacks expression of one of four Aminopeptidase N genes
Published in BMC genomics (24-06-2005)“…Insecticidal toxins from Bacillus thuringiensis bind to receptors on midgut epithelial cells of susceptible insect larvae. Aminopeptidases N (APNs) from…”
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Two different Bacillus thuringiensis toxin genes confer resistance to beet armyworm (Spodoptera exigua Hubner) in transgenic Bt-shallots (Allium cepa L.)
Published in Transgenic research (01-06-2005)“…Agrobacterium-mediated genetic transformation was applied to produce beet armyworm (Spodoptera exigua Hübner) resistant tropical shallots (Allium cepa L. group…”
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Lack of Detrimental Effects of Bacillus thuringiensis Cry Toxins on the Insect Predator Chrysoperla carnea: a Toxicological, Histopathological, and Biochemical Analysis
Published in Applied and Environmental Microbiology (01-02-2006)“…The effect of Cry proteins of Bacillus thuringiensis on the green lacewing (Chrysoperla carnea) was studied by using a holistic approach which consisted of…”
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The rin, nor and Cnr spontaneous mutations inhibit tomato fruit ripening in additive and epistatic manners
Published in Plant science (Limerick) (01-05-2020)“…•The spontaneous, gain-of-function alleles rin and nor function additively in repressing tomato fruit ripening.•The spontaneous Cnr mutant allele is epistatic…”
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Dominant negative phenotype of Bacillus thuringiensis Cry1Ab, Cry11Aa and Cry4Ba mutants suggest hetero-oligomer formation among different Cry toxins
Published in PloS one (16-05-2011)“…Bacillus thuringiensis Cry toxins are used worldwide in the control of different insect pests important in agriculture or in human health. The Cry proteins are…”
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