Cryopreservation of immature equine oocytes, comparing a solid surface vitrification process with open pulled straws and the use of a synthetic ice blocker

Cryopreservation of immature equine oocytes, comparing a solid surface vitrification process with open pulled straws and the use of a synthetic ice blocker

The objective was to evaluate the effect of three cryopreservation methods on the in vitro maturation (IVM) and membrane integrity (MIn) of immature equine oocytes. An open pulled straw (OPS) method, a novel solid surface vitrification (SSV) process, and the addition of a synthetic ice blocker were...

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Published in:Theriogenology Vol. 77; no. 1; pp. 21 - 27
Main Authors: de Leon, P.M.M, Campos, V.F, Corcini, C.D, Santos, E.C.S, Rambo, G, Lucia, T. Jr, Deschamps, J.C, Collares, T
Format: Journal Article
Language:English
Published: United States Elsevier Inc 2012
Subjects:
Animals
Cell Survival
cryopreservation
Cryopreservation - methods
Cryopreservation - veterinary
Cryoprotective Agents - pharmacology
Equine oocytes
Fertilization in Vitro - veterinary
Horses
ice
Ice blocker
IVM
Membrane integrity
oocytes
Oocytes - cytology
Oocytes - drug effects
Oocytes - ultrastructure
Vitrification
IVM
Ice blocker
Equine oocytes
Membrane integrity
Vitrification
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Summary:The objective was to evaluate the effect of three cryopreservation methods on the in vitro maturation (IVM) and membrane integrity (MIn) of immature equine oocytes. An open pulled straw (OPS) method, a novel solid surface vitrification (SSV) process, and the addition of a synthetic ice blocker were evaluated. Compared with the control group (N = 269), the OPS (N = 159) and the SSV (N = 202) cryopreservation methods decreased both IVM (50.9 vs. 13.3 and 9.4%, respectively; P < 0.001) and MIn (76.6 vs. 31.1 and 33.7%; P < 0.001) of immature equine oocytes. However, inclusion of 0.1% ice blocker in the OPS vitrification process increased the rates of both IVM (30.5%; P < 0.01) and MIn (45.8%; P < 0.05) of the oocytes (N = 59). Including 0.1% ice blocker in the SSV process improved the IVM rate (20.9%; P < 0.05), whereas MIn remained compromised in this group (N = 67). However, increasing the concentration of the ice blocker (to 1.0%) in the cryopreservation methods did not significantly improve rates of IVM. In conclusion, the addition of a synthetic ice blocker (0.1%) to both cryopreservation processes significantly increased rates of both IVM and MIn of immature equine oocytes cryopreserved by OPS.
Bibliography:http://dx.doi.org/10.1016/j.theriogenology.2011.07.008
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2011.07.008