Gastroprotective and ulcer healing effects of hydroethanolic extract of leaves of Caryocar coriaceum: Mechanisms involved in the gastroprotective activity

This work aimed to determine the chemical fingerprint of hydroethanolic extract of leaves of Caryocar coriaceum (HELCC) and the gastroprotective activity. The chemical fingerprint of HELCC was analyzed by HPLC-DAD, to quantify total phenols and flavonoids were carried out by Folin-Ciocalteu reagent...

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Published in:	Chemico-biological interactions Vol. 261; pp. 56 - 62
Main Authors:	de Lacerda Neto, Luis Jardelino, Ramos, Andreza Guedes Barbosa, Santos Sales, Valterlucio, de Souza, Severino Denicio Gonçalves, dos Santos, Antonia Thassya Lucas, de Oliveira, Larissa Rolim, Kerntopf, Marta Regina, de Albuquerque, Thais Rodrigues, Coutinho, Henrique Douglas Melo, Quintans-Júnior, Lucindo Jose, Wanderley, Almir Gonçalves, de Menezes, Irwin Rose Alencar
Format:	Journal Article
Language:	English
Published:	Ireland Elsevier Ireland Ltd 05-01-2017
Subjects:	Animals Antioxidant Antioxidants - metabolism Capsaicin - analogs & derivatives Capsaicin - pharmacology Capsaicin - therapeutic use Caryocar coriaceum Caryocaraceae Chemcial profile Chromatography, High Pressure Liquid Chronic Disease Disease Models, Animal Ericales - chemistry Ethanol - chemistry Female Flavonoids - analysis Gastrointestinal Motility Gastroprotection Glyburide - pharmacology Glyburide - therapeutic use Histamine - pharmacology Histamine - therapeutic use Indomethacin Male Mechanism of actions Mice Mucus - drug effects Naloxone - pharmacology Naloxone - therapeutic use NG-Nitroarginine Methyl Ester - pharmacology NG-Nitroarginine Methyl Ester - therapeutic use Phenols - analysis Phytotherapy Plant Extracts - pharmacology Plant Extracts - therapeutic use Plant Leaves - chemistry Protective Agents - therapeutic use Stomach Ulcer - drug therapy Stomach Ulcer - pathology Water - chemistry Yohimbine - pharmacology Yohimbine - therapeutic use Caryocar coriaceum Gastroprotection Antioxidant Mechanism of actions Chemcial profile Caryocaraceae
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Summary:	This work aimed to determine the chemical fingerprint of hydroethanolic extract of leaves of Caryocar coriaceum (HELCC) and the gastroprotective activity. The chemical fingerprint of HELCC was analyzed by HPLC-DAD, to quantify total phenols and flavonoids were carried out by Folin-Ciocalteu reagent and aluminum chloride assay, while in vitro antioxidant activity was evaluated by the DPPH method. The methods used to determine pharmacological activity were: gastroprotective screening test in classical models of induced acute and chronic gastric lesions and physical barrier test. Further assays were performed to demonstrate the involvement of NO, prostaglandins, ATP-dependent potassium channels, TRPV, noradrenergic α2 receptors, histamines, and opioids. The DPPH method demonstrated the antioxidant activity of the extract, in vitro, explained by the presence of polyphenols and flavonoids. Oral administration of the extract, previously dissolved in deionized water, at a dose of 100 mg/kg decreased the lesions induced by indomethacin, acidified ethanol, ethanol and acetic acid by 75.0, 72.8, 69.4 and 86.2% respectively. It was demonstrated that opioid receptors, α2-adrenergic receptors and primary afferent neurons sensitive to capsaicin were involved in the mechanism of gastric protection, in addition to the contribution of NO and prostaglandins. The results show that extract is a promising candidate for the treatment of gastric ulcers. [Display omitted] •The HELCC presents polyphenols and flavonoids.•Screening of activity of the extract for gastroprotection activity.•Elucidations of mechanism of actions for gastroprotection activity.•Histological analysis under chronic ulcer effect.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0009-2797 1872-7786
DOI:	10.1016/j.cbi.2016.11.020