Lipase Cocktail for Optimized Biodiesel Production of Free Fatty Acids from Residual Chicken Oil

In this work, the combination of different lipases was employed for the production of biodiesel, using the Free Fatty Acids (FFAs) from Residual Chicken Oil (RCO) as a substrate. As biocatalysts, lipases A and B from Candida antarctica (CALA and CALB, respectively) and lipase from Rhizomucor miehei...

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Bibliographic Details
Published in:	Catalysis letters Vol. 151; no. 4; pp. 1155 - 1166
Main Authors:	Rocha, Thales G., de L. Gomes, Pedro H., de Souza, Maria C. M., Monteiro, Rodolpho R. C., dos Santos, José C. S.
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-04-2021 Springer Springer Nature B.V
Subjects:	Biodiesel fuels Catalysis Chemistry Chemistry and Materials Science Esterification Ethanol Fatty acids Industrial Chemistry/Chemical Engineering Lipase Organometallic Chemistry Phenolphthalein Physical Chemistry Substrates Taguchi methods Biodiesel Residual chicken oil Lipase cocktail Taguchi method
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Summary:	In this work, the combination of different lipases was employed for the production of biodiesel, using the Free Fatty Acids (FFAs) from Residual Chicken Oil (RCO) as a substrate. As biocatalysts, lipases A and B from Candida antarctica (CALA and CALB, respectively) and lipase from Rhizomucor miehei (RML) were used in different combinations; as a result, the best lipase cocktail was composed of 67% CALB and 33% RML, which was used to optimize the esterification reaction parameters (molar ratio (FFAs/ ethyl alcohol), biocatalyst content, temperature and time) by the Taguchi method. Under optimized conditions (1:5, 15% of lipase cocktail, 30 °C and 3 h), it was possible to achieve 89.95 ± 0.3% of conversion. Graphic Abstract
ISSN:	1011-372X 1572-879X
DOI:	10.1007/s10562-020-03367-w