Effects of Injectable Solutions on the Quality of Monocyte-Derived Dendritic Cells for Immunotherapy

Effects of Injectable Solutions on the Quality of Monocyte-Derived Dendritic Cells for Immunotherapy

Therapeutic vaccines based on monocyte-derived dendritic cells have been shown to be promising strategies and may act as complementary treatments for viral infections, cancers, and, more recently, autoimmune diseases. Alpha-type-1-polarized dendritic cells (aDC1s) have been shown to induce type-1 im...

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Bibliographic Details
Published in:Journal of immunology research Vol. 2024; p. 6817965
Main Authors: Teodoro Da Silva, Laís, Tiaki Tiyo, Bruna, de Jesus Mota, Silvia, Mazzilli Ortega, Marina, Justamante Handel Schmitz, Gabriela, Nosomi Taniwaki, Noemi, Mitsue Namiyama Nishina, Gislene, José da Silva Duarte, Alberto, Miyuki Oshiro, Telma
Format: Journal Article
Language:English
Published: Egypt Hindawi Limited 07-06-2024
Wiley
Subjects:
Apoptosis
Autoimmune diseases
Cell Differentiation
Cell Survival - drug effects
Cell viability
Cells, Cultured
Clinical outcomes
Cloning
Cytokines
Cytokines - metabolism
Cytology
Dendritic cells
Dendritic Cells - immunology
Electron microscopy
Enzyme-linked immunosorbent assay
Flow cytometry
Granulocyte-macrophage colony-stimulating factor
Humans
Immunophenotyping
Immunotherapy
Immunotherapy - methods
Injections
Interleukin 12
Interleukin-12 - metabolism
Lactic acid
Microscopy
Monocytes
Monocytes - immunology
Morphology
Polyinosinic:polycytidylic acid
Quality control
Statistical analysis
Temperature
Tumor necrosis factor-α
Vaccines
Viral infections
α-Interferon
γ-Interferon
United States > US
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Summary:Therapeutic vaccines based on monocyte-derived dendritic cells have been shown to be promising strategies and may act as complementary treatments for viral infections, cancers, and, more recently, autoimmune diseases. Alpha-type-1-polarized dendritic cells (aDC1s) have been shown to induce type-1 immunity with a high capacity to produce interleukin-12p70 (IL-12p70). In the clinical use of cell-based therapeutics, injectable solutions can affect the morphology, immunophenotypic profile, and viability of cells before delivery and their survival after injection. In this sense, preparing a cell suspension that maintains the quality of aDC1s is essential to ensure effective immunotherapy. In the present study, monocytes were differentiated into aDC1s in the presence of IL-4 and GM-CSF. On day 5, the cells were matured by the addition of a cytokine cocktail consisting of IFN- , IFN- , IL-1 , TNF- , and Poly I:C. After 48 hr, mature aDC1s were harvested and suspended in two different solutions: normal saline and Ringer's lactate. The maintenance of cells in suspension was evaluated after 4, 6, and 8 hr of storage. Cell viability, immunophenotyping, and apoptosis analyses were performed by flow cytometry. Cellular morphology was observed by electron microscopy, and the production of IL-12p70 by aDC1s was evaluated by ELISA. Compared with normal saline, Ringer's lactate solution was more effective at maintaining DC viability for up to 8 hr of incubation at 4 or 22°C.
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Academic Editor: M. Victoria Delpino
ISSN:2314-8861
2314-7156
2314-7156
DOI:10.1155/2024/6817965