Functionally deficient neuronal differentiation of mouse embryonic neural stem cells in vitro

Embryonic mouse neural stem cells (NSCs) were isolated from E14 mice, multiplied in medium containing epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) and plated in laminin-coated wells in basic serum-free neurobasal medium. After 7 days in vitro, approximately 20% of the embr...

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Bibliographic Details
Published in:	Neuroscience research Vol. 49; no. 2; pp. 261 - 265
Main Authors:	Balasubramaniyan, V, de Haas, A.H, Bakels, R, Koper, A, Boddeke, H.W.G.M, Copray, J.C.V.M
Format:	Journal Article
Language:	English
Published:	Ireland Elsevier Ireland Ltd 01-06-2004
Subjects:	Animals Cell Differentiation - physiology Cells, Cultured Differentiation Electric Stimulation - methods Embryo, Mammalian Embryonic Epidermal Growth Factor - physiology Fibroblast Growth Factor 2 - physiology Glial Fibrillary Acidic Protein - physiology Immunohistochemistry - methods Membrane Potentials - physiology Membrane Potentials - radiation effects Mice Mice, Inbred C57BL Microtubule-Associated Proteins - metabolism Mouse Neural stem cells Neuron Neurons - cytology Neurons - physiology Patch-Clamp Techniques - methods Progenitor cells Stem Cells - cytology Stem Cells - physiology Embryonic Neural stem cells Neuron Mouse Differentiation Progenitor cells
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Summary:	Embryonic mouse neural stem cells (NSCs) were isolated from E14 mice, multiplied in medium containing epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) and plated in laminin-coated wells in basic serum-free neurobasal medium. After 7 days in vitro, approximately 20% of the embryonic mouse NSCs developed into morphologically and biochemically fully maturated neurons, with extensive dendrites and multiple synaptic contacts. However, even after 22 days of culture, none of these neurons developed voltage-dependent sodium-channels characteristic for a functional neuron. Apparently, the morphological differentiation and the electrophysiological maturation of an embryonic mouse NSC into a neuron are independently regulated.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0168-0102 1872-8111
DOI:	10.1016/j.neures.2004.02.010