NINscope, a versatile miniscope for multi-region circuit investigations

NINscope, a versatile miniscope for multi-region circuit investigations

Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural signals during unrestrained behavior and their open-source versions have made them affordable. Often, the footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present...

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Bibliographic Details
Published in:eLife Vol. 9
Main Authors: de Groot, Andres, van den Boom, Bastijn Jg, van Genderen, Romano M, Coppens, Joris, van Veldhuijzen, John, Bos, Joop, Hoedemaker, Hugo, Negrello, Mario, Willuhn, Ingo, De Zeeuw, Chris I, Hoogland, Tycho M
Format: Journal Article
Language:English
Published: England eLife Science Publications, Ltd 14-01-2020
eLife Sciences Publications Ltd
eLife Sciences Publications, Ltd
Subjects:
Accelerometers
Behavior
Brain
Brain research
Caudate-putamen
Cerebellum
Cerebral cortex
Design
Fluorescence
Fluorescence microscopy
imaging
Investigations
LEDs
Microscopes
miniscope
mouse
Neostriatum
Neuroscience
Semiconductor industry
Sensors
Software
striatum
Tools and Resources
United States
cerebral cortex
miniscope
mouse
striatum
neuroscience
imaging
cerebellum
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Summary:Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural signals during unrestrained behavior and their open-source versions have made them affordable. Often, the footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present NINscope: a light-weight miniscope with a small footprint that integrates a high-sensitivity image sensor, an inertial measurement unit and an LED driver for an external optogenetic probe. We use it to perform the first concurrent cellular resolution recordings from cerebellum and cerebral cortex in unrestrained mice, demonstrate its optogenetic stimulation capabilities to examine cerebello-cerebral or cortico-striatal connectivity, and replicate findings of action encoding in dorsal striatum. In combination with cross-platform acquisition and control software, our miniscope is a versatile addition to the expanding tool chest of open-source miniscopes that will increase access to multi-region circuit investigations during unrestrained behavior.
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ISSN:2050-084X
2050-084X
DOI:10.7554/elife.49987