Variation in mineral, organic, and water volumes at the neonatal line and in pre- and postnatal enamel

Variation in mineral, organic, and water volumes at the neonatal line and in pre- and postnatal enamel

•Enamel NNL in exfoliated primary incisors were studied.•Enamel component volumes were quantified at the NNL, pre- and post natal enamel.•Enamel component volumes variation against distance to the NNL were analyzed.•Prenatal enamel to NNL variations: -3.2 %(mineral), 35.4 % (organic), 6.0 % (water)....

Full description

Saved in:
Bibliographic Details
Published in:Archives of oral biology Vol. 118; p. 104850
Main Authors: de Andrade Dantas, Eugênia Lívia, de Figueiredo, Jonas Tostes, Macedo-Ribeiro, Natália, Oliezer, Renê Seabra, Gerlach, Raquel Fernanda, de Sousa, Frederico Barbosa
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-10-2020
Subjects:
Biochemistry
Dental Enamel - chemistry
Dental Enamel - embryology
Enamel
Female
Histology
Humans
Microradiography
Minerals
Neonatal line
Pregnancy
Tooth, Deciduous - chemistry
Water
Histology
Biochemistry
Enamel
Neonatal line
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Enamel NNL in exfoliated primary incisors were studied.•Enamel component volumes were quantified at the NNL, pre- and post natal enamel.•Enamel component volumes variation against distance to the NNL were analyzed.•Prenatal enamel to NNL variations: -3.2 %(mineral), 35.4 % (organic), 6.0 % (water).•Percentage differences from NNL to postnatal enamel followed the opposite trend. The neonatal line (NNL) in enamel is hypomineralized, but quantitative data on the enamel component volumes of the NNL are lacking. This study aimed at quantifying the variation in the mineral, organic, and water volumes at the NNL and in pre- and postnatal enamel. In buccal enamel longitudinal ground sections of exfoliated primary incisors (upper and lower; n = 17), the enamel component volumes were quantified at five histological sites (located at 40 μm intervals along a transversal line): the NNL, two sites in prenatal enamel, and two sites in postnatal enamel. Mineral volume was quantified using microradiography, and non-mineral volumes were quantified using polarizing microscopy. Differences in component volumes between the NNL and pre- and postnatal enamel had high effect sizes (Hedge’s G ranging from 0.89, for the water volume, to 1.88, for the mineral volume; power > 90 %). The distance from the NNL correlated with the normalized component volume: r = 0.459, 95 % CI = 0.274/0.612 (mineral); r = -0.504; 95 % CI= -0.328/-0.647 (organic), and r = -0.294; 95 % CI= -0.087/-0.476 (water). Approaching the NNL from postnatal enamel, the percentage differences in component volumes were: -1.93 to -3.22 % for the mineral volume, +21.26 to +35.42 % for the organic volume, and +3.86 to +6.03 % for the water volume. Towards postnatal enamel, the percentage differences had the opposite trend. The enamel NNL is slightly hypomineralized with an increased organic volume one order of magnitude higher than the percentage differences in both mineral and water volumes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2020.104850