Profiles of Staphyloccocus aureus isolated from goat persistent mastitis before and after treatment with enrofloxacin

Profiles of Staphyloccocus aureus isolated from goat persistent mastitis before and after treatment with enrofloxacin

Staphylococcus aureus is one of the main causative agents of mastitis in small ruminants. Antimicrobial use is the major treatment, but there are many flaws linked to resistance, tolerance or persistence. This study aimed to verify changes in resistance, virulence and clonal profiles of S. aureus is...

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Published in:BMC microbiology Vol. 20; no. 1; p. 127
Main Authors: Lima, Magna Coroa, de Barros, Mariana, Scatamburlo, Thalita Moreira, Polveiro, Richard Costa, de Castro, Laís Karolyne, Guimarães, Samuel Henrique Sales, da Costa, Sanely Lourenço, da Costa, Mateus Matiuzzi, Moreira, Maria Aparecida Scatamburlo
Format: Journal Article
Language:English
Published: England BioMed Central Ltd 24-05-2020
BioMed Central
BMC
Subjects:
Animals
Antiinfectives and antibacterials
Antimicrobial agents
Bacteria
Biotechnology industries
Caprine mastitis
Cell division
Comparative analysis
Efflux
Enrofloxacin
Flaw detection
Gene expression
Genes
Goat's milk
Goats
Gram-positive bacteria
Mammary gland
Mammary glands
Mastitis
Milk
Minimum inhibitory concentration
Oxacillin
Pathogens
Penicillin
Persistence
Plasmids
Small ruminants
Staphylococcal enterotoxin H
Staphylococcal enterotoxin J
Staphylococcus aureus
Staphylococcus infections
Tetracyclines
Virulence
Virulence (Microbiology)
United States
Persistence
Caprine mastitis
Small ruminants
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Summary:Staphylococcus aureus is one of the main causative agents of mastitis in small ruminants. Antimicrobial use is the major treatment, but there are many flaws linked to resistance, tolerance or persistence. This study aimed to verify changes in resistance, virulence and clonal profiles of S. aureus isolated from persistent mastitis goat milk before and after enrofloxacin treatment. MIC increased to at least one antimicrobial in S. aureus isolates after enrofloxacin treatment compared to before. The most detected resistance genes before and after treatment were tetK, tetM, and blaZ, with more resistance genes detected after enrofloxacin treatment (p < 0.05). Occasional variations in efflux system gene detection were observed before and after treatment. Nine virulence genes (hla, fnbA, fnbB, eta, etb, sea, sec, seh, and sej) were detected at both times, and between these, the hla and eta genes were detected more in isolates after treatment. All isolates of S. aureus belonged to the same sequence type (ST) 133, except for two S. aureus isolates prior to enrofloxacin treatment which were classified as ST5 and the other as a new one, ST4966. Isolates of S. aureus 4, 8, and 100 from before and after treatment had identical pulse types, while others obtained from other animals before and after treatment were classified into distinct pulse types. There were occasional changes in the studied profiles of S. aureus isolated before and after treatment of animals with enrofloxacin, which may have contributed to the permanence of bacteria in the mammary gland, even when using traditional treatment, resulting in persistent mastitis.
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ISSN:1471-2180
1471-2180
DOI:10.1186/s12866-020-01793-9