Degradation of crystallins from a psoriatic patient undergoing PUVA therapy

Comparative physico-chemical and spectroscopic analyses were carried out in human lens proteins obtained from extracts of normal, senile and PUVA cataracts. Mass recovery analysis reveals a large protein concentration loss in the PUVA cataract relative to the normal lens and senile cataract. This pr...

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Bibliographic Details
Published in:	FEBS letters Vol. 268; no. 1; pp. 72 - 74
Main Authors:	e Melo, Teresa Sa, de Castro, José Cime, Ribeiro, Luisa, Conte, Joao, Lawrence, David, Bazin, Marc, Santus, René
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 30-07-1990 Elsevier
Subjects:	8-Methoxypsoralen 8MOP, 8-methoxypsoralen Aging Biological and medical sciences Biological effects of radiation Cataract - etiology Cataract - physiopathology Crystallins - metabolism Eye Proteins - analysis Fundamental and applied biological sciences. Psychology Humans Lens fluorescence Lens protein Lens, Crystalline - metabolism Molecular Weight Photosensitizer psoralen psoriasis Psoriasis - therapy PUVA cataract PUVA Therapy - adverse effects PUVA, psoralen + U VA Radiosensitizing agents. Photosensitizing agents. Thermosensitizing agents Solubility Spectrometry, Fluorescence Tissues, organs and organisms biophysics Trp, tryptophan Tryptophan Tryptophan - analysis U.V. radiation 8MOP, 8-methoxypsoralen PUVA, psoralen + U VA Trp, tryptophan Tryptophan 8-Methoxypsoralen Photosensitizer PUVA cataract Lens protein Lens fluorescence Human Proteins Cataract Psoriasis Lens PUVA therapy Physicochemical properties Fluorescence spectrometry Ultraviolet visible spectrometry
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Summary:	Comparative physico-chemical and spectroscopic analyses were carried out in human lens proteins obtained from extracts of normal, senile and PUVA cataracts. Mass recovery analysis reveals a large protein concentration loss in the PUVA cataract relative to the normal lens and senile cataract. This protein loss parallels an increase in the degraded polypeptide chains. However, the tryptophan content (2.1 mol mol of 20 kDa protein subunit) and the apparent fluorescence quantum yield (ϕ f = 0.056) of the tryptophan residues which are believed to be involved in the development of UV-induced cataracts are unchanged after age-related alterations and/or in vivo photochemistry associated with psoralen (8MOP) photosensitized reactions.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(90)80975-O